摘要
为了开发应用与脱去黄瓜果面蜡粉能力密切相关的砧用南瓜分子标记,对18份脱蜡粉能力不同的砧用南瓜材料进行嫁接评比。结果发现,脱蜡粉能力强的南瓜作为砧木与黄瓜嫁接后,接穗黄瓜的茎、叶及果实中的硅含量显著低于脱蜡粉能力弱的南瓜砧木,说明砧用南瓜的脱蜡粉能力与其对硅元素的吸收转运能力有关。进一步使用基于硅转运蛋白基因CmLSi1的SNP开发的已报道分子标记CM1-CAPS以及本研究设计的SNP-Sanger测序和Hi-Tom测序方法,鉴定和分析57份脱蜡粉能力不同的砧用南瓜材料。结果表明,3种鉴定方法对脱蜡粉性状的选择准确率都达到100%,说明该分子标记适用于脱蜡粉砧用南瓜品种选育工作,对加速新品种选育具有重要意义。
To develop molecular markers for Cucurbita maxima rootstocks related to their ability to remove cucumber fruit surface’s bloom,evaluated 18 different C.maxima materials with varying bloom removal capabilities through grafting.The results showed that when cucumber was grafted onto rootstocks with strong bloom removal abilities,the silicon content in the stems,leaves,and fruits of the scion cucumber was significantly lower compared to those grafted onto rootstocks with weak bloom removal abilities.This indicates that the bloom removal capability of C.maxima is associated with its ability to absorb and transport silicon.Using the previously reported molecular marker CM1-CAPS based on SNP of the silicon transporter gene CmLSi1,along with the SNP-Sanger sequencing and Hi-Tom sequencing methods designed in this study,we identified and analyzed 57 C.maxima rootstocks with varying abilities to bloom removing.The results showed that all three identification methods achieved a 100% accuracy rate for selecting the bloom removing trait,indicating that this molecular marker is suitable for breeding bloom removing rootstocks,which is of great significance to accelerate the breeding of new varieties.
作者
裴轶凡
郑秀
别之龙
周玉忠
王忠全
成金桃
PEI Yifan;ZHENG Xiu;BIE Zhilong;ZHOU Yuzhong;WANG Zhongquan;CHENG Jintao(National Key Laboratory of Germplasm Innovation and Utilization of Fruit and Vegetable Horticultural Crops/College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430070,Hubei,China;Qingdao Jinmama Seed Technology Co.,Ltd.,Qingdao 266611,Shandong,China)
出处
《中国瓜菜》
北大核心
2025年第10期39-47,共9页
China Cucurbits And Vegetables
基金
国家重点研发计划项目(2023YFD2300703)
山东省科技型中小企业创新能力提升工程项目(2022TSGC1143)
青岛市创新创业共同体项目(22-7-5-gtt-6-gx)
果蔬园艺作物种质创新与利用全国重点实验室委托项目(Horti-3Y-2024-014)。
关键词
砧用南瓜
黄瓜
脱蜡粉
分子标记
硅转运蛋白
Rootstock pumpkin
Cucumber
Bloom removal
Molecular marker
Silicon transporter protein
