摘要
研究高能电子束辐照对人工栽培灵芝多糖(GLP)理化性质的影响及其抗运动疲劳活性。GLP通过水提醇沉法提取,并通过离子交换层析和分子排阻色谱纯化。采用不同剂量的高能电子束辐照处理GLP,并利用扫描电镜和红外光谱进行理化性质表征。通过负重游泳构建运动疲劳大鼠模型,灌胃GLP,验证其抗运动疲劳效果。结果显示:GLP重均分子质量1.87×10^(6),平均粒径779.42 nm,异头碳为β构型。随着辐照剂量增加,GLP相对分子质量和粒径先增大后减小,表现出先交联后解聚的变化。20 kGy辐照处理的GLP显著提高了疲劳大鼠的肝糖原含量(增加31.37%)和肌糖原含量(增加52.94%),同时降低了尿素氮含量(降低41.67%)和血清乳酸含量(降低43.75%)。此外,辐照处理后的GLP能够增强大鼠线粒体内源性抗氧化酶活性并减少丙二醛积累。与阳性对照(红景天苷)相比,20 kGy辐照处理的GLP表现出更好的抗疲劳效果。因此,适宜剂量的高能电子束辐照是一种改性GLP的理想方法。
The effects of high-energy electron beam irradiation on the physicochemical properties and antifatigue activity of cultivated Ganoderma lingzhi polysaccharides(GLPs)were investigated.GLPs were extracted using the water extraction and alcohol precipitation method and subsequently purified through ion exchange and gel filtration chromatography.Different doses of high-energy electron beam irradiation were applied to GLPs,and their physicochemical properties were characterized using scanning electron microscopy and infrared spectroscopy.A rat model of exercise fatigue was established through weight-loaded swimming,and GLPs were administered via gavage to verify their anti-fatigue effects.The results showed that the weight-average molecular weight of GLPs was 1.87×10^(6),with an average particle size of 779.42 nm,and the anomeric carbon was in theβ-configuration.As the irradiation dose increased,the molecular weight and particle size of GLPs initially increased and subsequently decreased,indicating a transition from cross-linking to depolymerization.GLPs treated with 20 kGy irradiation was found to significantly increase the liver and muscle glycogen contents(by 31.37%and 52.94%,respectively)while decrease the blood urea nitrogen and serum lactic acid contents(by 41.67%and 43.75%,respectively)in fatigued rats.Additionally,the irradiated GLPs enhanced the endogenous antioxidant enzyme activity in rat mitochondria and reduced malondialdehyde accumulation.Compared with the positive control(Rhodiola rosea glycoside),GLPs treated with 20 kGy irradiation demonstrated superior anti-fatigue effects.Therefore,an appropriate dose of highenergy electron beam irradiation was deemed an ideal method for modifying GLPs.
作者
肖东林
王蓉
王怀虎
魏玉琴
XIAO Donglin;WANG Rong;WANG Huaihu;WEI Yuqin(College of Physical Education,Xi'an Shiyou University,Xi'an 710065,China;College of Chemical Engineering,Xi'an Shiyou University,Xi'an 710065,China;PE Department of Tianshui School of Sport and Health,Tianshui 741001,China)
出处
《现代食品科技》
北大核心
2025年第9期11-21,共11页
Modern Food Science and Technology
基金
陕西省教育厅专项科研计划项目(22JK0148)
陕西省高等教育教学改革研究项目(23ZY016)
西安石油大学校级教育教学改革研究项目(JGQZ202303)
天水师范学院科研创新基金专项(CXJ2023-09)。
关键词
灵芝
多糖
结构分析
高能电子束辐照
抗运动疲劳
Ganoderma spp.
polysaccharides
structural analysis
high-energy electron beam irradiation
anti-fatigue activity
