摘要
目的分析前列腺癌患者血清微小RNA(miR)-425-5p、miR-221变化及与术后复发转移的关系。方法回顾性分析病例资料选自南京医科大学附属无锡人民医院2020年9月至2023年12月收治的95例前列腺癌患者,根据术后复发转移情况分组,将存在术后复发转移的38例患者纳入A组,将未存在复发转移的57例患者纳入B组,并选取同期健康体检者80例纳入对照组。观察三组患者血清miR-425-5p、miR-221水平差异,并绘制受试者工作(ROC)曲线分析上述指标与术后复发转移的预测能力。结果三组间血清miR-425-5p、miR-221表达水平比较差异有统计学意义(P<0.05)。三组间血清miR-425-5p、miR-221表达水平比较:A组>B组>对照组,差异有统计学意义(P<0.05)。A组患者在临床分期、Gleason评分、入院时PSA水平高于B组患者,差异具有统计学意义(P<0.05),经Logisitic多因素回归分析,临床分期为Ⅲa、Ⅲb期、Gleason评分>7分、Gleason评分为4+3分、血清miR-425-5p和miR-221表达水平高是前列腺癌患者术后复发转移的危险因素(P<0.05)。绘制ROC曲线发现,血清miR-425-5p、miR-221表达水平对前列腺癌患者术后复发转移预测的ROC曲线下面积(AUC)分别为0.842、0.849,联合预测AUC最高为0.940。结论前列腺癌患者血清miR-425-5p和miR-221的表达水平均呈升高趋势,对前列腺癌术后复发转移具有一定的预测价值。
Objective To analyze the changes of serum microRNA(miR)-425-5p and miR-221 in patients with prostate cancer and the relationship with postoperative recurrence/metastasis.Methods A retrospective analysis was conducted on case data of 95 patients with prostate cancer admitted to Wuxi People's Hospital Affiliated to Nanjing Medical University from September 2020 to December 2023.Based on the postoperative recurrence/metastasis status,38 patients with postoperative recurrence/metastasis were included in group A,while 57 patients without recurrence/metastasis were placed in group B.Additionally,80 healthy subjects who underwent physical examinations were selected as the control group.Differences in serum miR-425-5p and miR-221 levels were observed among the three groups of patients,and a receiver operating characteristic(ROC)curve was drawn to analyze the predictive ability of these indicators for postoperative recurrence/metastasis.Results There were statistical differences in the expression levels of serum miR-425-5p and miR-221 among the three groups(P<0.05).Serum miR-425-5p and miR-221 were expressed as group A>group B>control group,with statistical differences(P<0.05).The clinical staging,Gleason score and PSA level at admission in group A were higher than those in group B,with statistical differences(P<0.05).Logistic multivariate regression analysis showed that clinical stage Ⅲa and Ⅲb,Gleason score>7 points,Gleason scored 4+3 points,and high expression levels of serum miR-425-5p and miR-221 were risk factors for postoperative recurrence/metastasis in prostate cancer patients(P<0.05).Drawing a ROC curve found that the areas under the ROC curves(AUCs)of serum miR-425-5p and miR-221 expression levels for predicting postoperative recurrence/metastasis were 0.842 and 0.849,respectively,and the AUC of combined prediction was the highest at 0.940.Conclusion The levels of serum miR-425-5p and miR-221 in patients with prostate cancer show increasing trends and have a certain predictive value for postoperative recurrence/metastasis of prostate cancer.
作者
黄劲熹
唐昊
胡强
HUANG Jingxi;TANG Hao;HU Qiang(Department of Urological Surgery,Wuxi People's Hospital Affiliated to Nanjing Medical University,Wuxi,Jiangsu,China,214023)
出处
《分子诊断与治疗杂志》
2025年第10期1896-1899,共4页
Journal of Molecular Diagnostics and Therapy
基金
江苏省科技计划专项基础研究计划自然科学基金项目(BK20221200)。
