摘要
以金耳(Naematelia aurantialba)T-1孢子为试验材料,对金耳的生物学特性进行探究并对其进行全基因组测序及分析。结果表明,T-1的最佳碳源为蔗糖,最佳氮源为蛋白胨,最佳无机盐为氯化钾,最适宜pH为7,最佳接种量为10^(8)个细胞/mL;基因组碱基测序质量为35~40,测序质量较好;碱基的AT、GC含量较为均一,测序结果正常;利用K-mer频率分析出T-1基因组大小为26 612 159 bp;对测序数据拼接后显示N50较高,GC含量为56.45%;使用Circos软件对基因组进行展示表明,T-1基因组全长为18180 385 bp,GC含量为56.46%,基因组共被分为10个contig;系统发育树显示T-1与金耳菌株聚类关系最近;GO功能分类显示被注释到3大类别中,其中参与生物学过程的基因最多;与KEGG数据库比对后,共4 096个基因被注释到五大类代谢途径,其中参与代谢途径的基因最多。
The spores of Naematelia aurantialba T-1 were used as the experimental material.The biological characteristics of Naematelia aurantialba were studied,and its whole genome was sequenced and analyzed.The results indicated that the optimal carbon source for T-1 was sucrose,the optimal nitrogen source was peptone,the optimal inorganic salt was potassium chloride,the most suitable pH was 7,and the optimal inoculation amount was 10^(8) cells/mL.The sequencing quality of the genomic bases was between 35 and 40,which was relatively good.The AT and GC base contents were relatively uniform,and the sequencing results were normal.Through K-mer frequency analysis,the size of the T-1 genome was determined to be 26612159 bp.After the sequencing data were assembled,it was shown that the N50 value was relatively high,and the GC content was 56.45%.The display of the genome using Circos software indicated that the full-length of the T-1 genome was 18180385 base pairs,with a GC content of 56.46%.The genome was divided into 10 contigs.The phylogenetic tree showed that T-1 was most closely clustered with Naematelia aurantialba strains.The GO function classification was annotated into three major categories,among which the genes involved in biological processes were the most.After comparison with the KEGG database,a total of 4096 genes were annotated into five major metabolic pathways,among which the genes involved in metabolic pathways were the most.
作者
田爽
毕宇
郭燕
吴皓琼
叶阳
刘佳宁
朱加楠
TIAN Shuang;BI Yu;GUO Yan;WU Hao-qiong;YE Yang;LIU Jia-ning;ZHU Jia-nan(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,China)
出处
《湖北农业科学》
2025年第8期152-159,共8页
Hubei Agricultural Sciences
基金
黑龙江省属科研院所科研业务费项目(2023SSKY004-04)。
