摘要
目的筛选黑曲霉及其混合菌株固态发酵黄芩茎叶的条件,促进黄芩茎叶中野黄芩苷向其苷元野黄芩素高效转化,为黄芩茎叶高值化利用提供可行技术方案。方法分别采用黑曲霉、枯草芽孢杆菌和产朊假丝酵母固态发酵黄芩茎叶,检测野黄芩苷和野黄芩素以评估效果。采用不同培养基料水比、黑曲霉种子液接种量、发酵温度、固态发酵时间进行发酵实验,检测纤维素酶活性、野黄芩苷和野黄芩素,筛选黑曲霉固态发酵黄芩茎叶的最佳条件。将不同比例的枯草芽孢杆菌、黑曲霉、产朊假丝酵母混合菌株种子液发酵黄芩茎叶,筛选最佳混合菌株接种比例,进而筛选最佳接种量、发酵时间。结果当以黑曲霉发酵黄芩茎叶时,发酵培养料水比1∶1.5,菌液接种量20%,发酵温度35℃,发酵时间9 d,发酵样品中野黄芩素含量最高;当以混合菌株发酵黄芩茎叶时,枯草芽孢杆菌∶黑曲霉∶产朊假丝酵母为1∶1∶2,混合菌株接种量(以黑曲霉计)15%,发酵时间9 d,发酵样品中野黄芩素含量最高。结论黑曲霉及其混合菌株均可显著促进黄芩茎叶中野黄芩苷向野黄芩素高效转化,当枯草芽孢杆菌∶黑曲霉∶产朊假丝酵母为1∶1∶2、混合菌株接种量为15%、发酵时间为9 d时转化效果最好。
Objective To optimize the conditions for solid-state fermentation of Scutellaria baicalensis(S.baicalensis)stems and leaves using Aspergillus niger and its mixed microbial consortia,so as to promote the efficient biotransformation of baicalin into its aglycone baicalein,and thereby to provide a scientific basis for the high-value utilization of S.baicalensis stems and leaves.Methods Solid-state fermentation of S.baicalensis stems and leaves was performed separately using A.niger,Bacillus subtilis,and Candida utilis,with baicalin and baicalein contents determined to evaluate fermentation efficiency.Fermentation experiments were conducted under different substrate-to-moisture ratios,inoculum sizes of A.niger seed culture,incubation temperatures,and fermentation durations.Cellulase activity,baicalin,and baicalein levels were measured to determine the optimal fermentation conditions for A.niger.Subsequently,mixed inoculations with different ratios of B.subtilis,A.niger,and C.utilis were tested,followed by optimization of inoculum size and fermentation duration to identify the most effective mixed-strain combination.Results For A.niger fermentation,the highest baicalein yield was obtained under the conditions of a substrate-to-moisture ratio of 1:1.5,an inoculum size of 20%,incubation at 35°C,and a fermentation period of 9 days.For mixed-strain fermentation,the optimal ratio of B.subtilis:A.niger:C.utilis was 1:1:2,with a total inoculum size of 15%(based on A.niger)and a fermentation duration of 9 days,resulting in the highest baicalein content.Conclusions Both A.niger and its mixed microbial consortia significantly enhanced the bioconversion of baicalin to baicalein in S.baicalensis stems and leaves.When the inoculum ratio of B.subtilis:A.niger:C.utilis was 1:1:2,the inoculation volume was 15%,and the fermentation time was 9 days,the conversion effect was the best.
作者
宿晓蓉
许朝且
SU Xiaorong;XU Chaoqie(Department of Pharmacy,Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine,Nanjing 210029,China)
出处
《山东医药》
2025年第9期117-121,共5页
Shandong Medical Journal
基金
国家重点研发计划(2017YFD0501502)
江苏省中药资源产业化过程协同创新中心开放课题(0120902002006-27)。
关键词
微生物发酵
固态发酵
黄芩茎叶
野黄芩苷
野黄芩素
microbial fermentation
solid-state fermentation
Scutellaria baicalensis stems and leaves
scutellarin
scutellarein
