摘要
针对常规饮用水处理中的蓝藻及其代谢产物的去除难题,向含藻原水中添加不同类型的混凝剂(壳聚糖、聚合氯化铝PAC及三氯化铁FeCl_(3)),探讨了混凝剂对蓝藻细胞破损及微囊藻毒素LR(MC-LR)厌氧生物降解的影响。采用3D-EEM荧光谱、扫描电子显微(SEM)及酶联免疫吸附测定(ELISA)技术进行了分析。研究结果表明,FeCl_(3)的混凝絮体对蓝藻细胞破损及总MC-LR降解未表现出明显影响;壳聚糖在初期具有保护蓝藻细胞和吸附胞外MC-LR的效果,但随着时间推移,壳聚糖的功能逐渐减弱;而PAC则显著加剧蓝藻细胞破损,导致胞内毒素大量释放,从而加快了总MC-LR的降解速率。同时,通过3D-EEM荧光图谱检测到氨酸/色氨酸蛋白区、酪氨酸/色氨酸区等5类物质质量浓度随反应进程增加,表明不同混凝剂对底泥中胞外聚合物(EPS)的产生有显著影响,且PAC组中相关物质增加最快。该研究系统探讨了混凝剂对蓝藻细胞及MC-LR厌氧降解的影响机制,为给水污泥中蓝藻及其毒素的高效去除提供了重要参考依据。
This study investigated the effects of different coagulants—namely chitosan,polyaluminum chloride(PAC),and ferric chloride(FeCl_(3))—on cyanobacterial cell breakage and microcystin-LR(MC-LR)anaerobic biodegradation in raw water containing algae during conventional drinking-water treatment.Analyses were conducted using three-dimensional excitation emission matrix(3D-EEM)fluorescence spectroscopy,scanning electron microscopy,and enzyme-linked immunosorbent assays.Results showed that coagulated flocs generated by the action of FeCl_(3)had no significant effect on cyanobacterial cell breakage or total MC-LR degradation.Chitosan initially protected cyanobacterial cells and adsorbed extracellular MC-LR;however,its effectiveness declined over time.Meanwhile,PAC significantly exacerbated cyanobacterial cell breakage,leading to the massive release of intracellular toxins,which ultimately accelerated the degradation rate of total MC-LR.In addition,3D-EEM fluorescence spectroscopy revealed increasing concentrations of five substance types,such as the ammoniacal/tryptophan protein zone and tyrosine/tryptophan zone,indicating that the different coagulants had a significant effect on the production of extracellular polymers in the sludge.Further,the related substances increased in amount the fastest when PAC was used.This study is the first to systematically investigate the mechanisms by which coagulants affect cyanobacterial cells and MC-LR anaerobic degradation,providing an important reference for the efficient removal of cyanobacteria and their toxins from waterworks sludge.
作者
马广翔
鞠甜甜
夏延钊
陈琳琳
宋汶阳
刘越
MA Guangxiang;JU Tiantian;XIA Yanzhao;CHEN Linlin;SONG Wenyang;LIU Yue(Shandong Wolan Environmental Technology Co.,Ltd,Jinan 250014,China;Shandong University,Jinan 250100,China;Shandong Society of Environmental Sciences,Jinan 250014,China;Shandong Provincial Construction Project Environmental Review Service Center,Jinan 250011,China)
出处
《山东科学》
2025年第5期123-130,共8页
Shandong Science
基金
山东省重点研发计划(国际科技合作)项目(2019GHZ030)。
关键词
给水污泥
微囊藻毒素LR
混凝剂絮体
含藻底泥
厌氧生物降解
waterworks sludge
microcystin-LR
coagulant flocs
alga-containing sludge
anaerobic biodegradation
