摘要
目的探讨γ干扰素诱导蛋白10(interferonγ-inducible protein-10,IP-10)、白细胞介素-2(interleukin-2,IL-2)、单核细胞趋化因子-1(monocyte chemotactic protein 1,MCP-1)、干扰素-γ(interferon-γ,INF-γ)血液浓度检测在活动性肺结核(active tuberculosis,ATB)诊断中的应用。方法回顾性分析2023年11月~2024年11月在洪雅县中医医院就诊的110例ATB患者的临床资料作为观察组,另选择同期90例其他肺部疾病患者作为肺病组,60例健康体检者作为对照组。对比3组的IP-10、IL-2、MCP-1、INF-γ血液浓度,分析IP-10、IL-2、MCP-1、INF-γ与ATB的关系,绘制受试者工作特征(ROC)曲线探讨IP-10、IL-2、MCP-1、INF-γ诊断ATB的价值。结果观察组的IP-10、IL-2、MCP-1、INF-γ高于肺病组、对照组(P<0.05);肺病组的IP-10、IL-2、MCP-1、INF-γ高于对照组(P<0.05)。ROC曲线分析显示IP-10、IL-2、MCP-1、INF-γ联合诊断ATB的效能(AUC:0.813,95%CI:0.784~0.842)优于IP-10(AUC:0.663,95%CI:0.645~0.681)、IL-2(AUC:0.627,95%CI:0.611~0.643)、MCP-1(AUC:0.721,95%CI:0.685~0.757)、INF-γ(AUC:0.734,95%CI:0.692~0.776)单一指标(P<0.05)。Logistic分析显示IP-10升高(OR:3.435,95%CI:1.874~4.996)、IL-2升高(OR:1.314,95%CI:1.264~6.178)、MCP-1升高(OR:3.691,95%CI:2.521~5.561)、INF-γ升高(OR:3.710,95%CI:1.584~5.836)是ATB发生的独立危险因素(P<0.05)。结论IP-10、IL-2、MCP-1、INF-γ在ATB中呈高表达,IP-10、IL-2、MCP-1、INF-γ水平升高为ATB发生的危险因素,将IP-10、IL-2、MCP-1、INF-γ联用于ATB诊断中能提高结果的准确性。
Objective To explore the application of serum interferon-gamma-induced protein 10(IP-10),interleukin-2(IL-2),monocyte chemoattractant protein-1(MCP-1),and interferon-gamma(INF-γ)in the diagnosis of active tuberculosis(ATB).Methods The clinical data of 110 ATB patients who visited Hongya County Traditional Chinese Medicine Hospital from November 2023 to November 2024 were retrospectively analyzed as the observation group.Another 90 patients with other lung diseases and 60 healthy individuals undergoing physical examinations during the same period were selected as the lung disease group and the control group,respectively.The levels of serum IP-10,IL-2,MCP-1 and INF-γin the three groups were compared.The relationship between IP-10,IL-2,MCP-1,INF-γand ATB was analyzed,and the receiver operating characteristic(ROC)curve was drawn to explore the diagnostic value of IP-10,IL-2,MCP-1,and INF-γfor ATB.Results The levels of IP-10,IL-2,MCP-1,and INF-γin the observation group were higher than those in the lung disease group and the control group(P<0.05);the levels of IP-10,IL-2,MCP-1,and INF-γin the lung disease group were higher than those in the control group(P<0.05).ROC curve analysis showed that the combined diagnosis of ATB with IP-10,IL-2,MCP-1,and INF-γ(AUC:0.813,95%CI:0.784-0.842)was superior to the single indicators of IP-10(AUC:0.663,95%CI:0.645-0.681),IL-2(AUC:0.627,95%CI:0.611-0.643),MCP-1(AUC:0.721,95%CI:0.685-0.757),and INF-γ(AUC:0.734,95%CI:0.692-0.776)(P<0.05).Logistic analysis revealed that elevated IP-10(OR:3.435,95%CI:1.874-4.996),elevated IL-2(OR:1.314,95%CI:1.264-6.178),elevated MCP-1(OR:3.691,95%CI:2.521-5.561),and elevated INF-γ(OR:3.710,95%CI:1.584-5.836)were independent risk factors for the occurrence of ATB(P<0.05).Conclusion IP-10,IL-2,MCP-1,and INF-γare highly expressed in ATB,and elevated levels of IP-10,IL-2,MCP-1,and INF-γare risk factors for the occurrence of ATB.The combined use of IP-10,IL-2,MCP-1,and INF-γin the diagnosis of ATB can improve the accuracy of the results.
作者
王波
韩伟
WANG Bo;HAN Wei(Department of Tuberculosis,First Affiliated Hospital of Xinxiang Medical College,Xinxiang,Henan,453000,China;Deparment of Pulmonary and Critical Care Medicine,Hongya County Traditional Chinese Medicine Hospital,Meishan,Sichuan,620360,China)
出处
《医学分子生物学杂志》
2025年第5期476-481,共6页
Journal of Medical Molecular Biology
基金
河南省医学科技攻关计划项目(No.LHGJ20220594)。
