摘要
目的用RNA恒温扩增实时检测（SAT—TB）快速检测临床痰标本中的MTB，并对检测效果进行评估。方法痰标本取自上海市肺科医院的肺结核患者177例（肺结核组）和其他肺部疾病患者67例（对照组），采用SAT．TB检测和罗氏培养法同时对244份痰标本进行检测，用MTB核酸扩增荧光体外检测试剂盒（PCR—TB）检测2种检测方法结果不符的标本。分析SAT—TB与罗氏培养的符合率及两种方法对结核病患者的检出率。两种检测方法的检出率比较采用χ2检验。结果以罗氏培养作为金标准，则SAT．TB检测的敏感度和特异度分别为92％（71／77）和86％（143／167），SAT—TB检测与罗氏培养结果的符合率为88％（214／244）。罗氏培养法和SAT—TB检测对临床确诊的肺结核患者痰标本的阳性检出率分别为42％（75／177）和54％（95／177），两者的阳性率比较，差异有统计学意义（χ2=4．527，P〈0．05）。结论SAT—TB检测痰标本中的MTB，具有快速、敏感度和特异度较高的优点，可提高痰标本中MTB的检出率。
Objective To evaluate the use of isothermal RNA amplification assay for detection of Mycobacterium tuberculosis （SAT-TB） in sputum samples. Methods A total of 244 sputum samples from patients with pulmonary tuberculosis and those with other lung diseases were detected by SAT-TB and Lowenstein-Jensen （L-J） culture. The samples with different results between SAT-TB and L-J culture were tested by Mycobacterium tuberculosis PCR fluorescence diagnostic kits. The sensitivity and specificity of SAT- TB were calculated according to the results of L-J culture. The detection rates of SAT-TB and L-J culture were analyzed according to the clinical diagnosis and the difference of the 2 methods were analyzed by chi- square test. Results With the result of L-J cuhure as the reference, the sensitivity and the specificity of SAT-TB were 92% （71/77）and 86% （143/167） , respectively. The accordance rate of SAT-TB and L-J culture was 88% （214/244）. For tuberculosis patients, the detection rate of L-J culture and SAT-TB was 42% （75/177） and 54% （95/177） respectively. The difference between the detection rates of SAT-TB and L-J was significant by chi-square test （ χ2 = 4. 527, P 〈 0. 05 ）. Conclusions SAT-TB is a rapid, sensitive and specific method for detection of Myeobacterium tuberculosis in clinical sputum samples.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2011年第12期894-897,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
上海市科学技术委员会科研计划项目课题（10411955100、10QA1405800）
关键词
分枝杆菌
结核
核酸扩增技术
痰
诊断
Mycobacterium tuberculosis
Nucleic acid amplification techniques
Sputum
Diagnosis
