摘要
目的基于AMPK/SIRT1/PGC-1α信号通路观察针刺“夹脊”对腰肌衰老模型大鼠血管生成的影响,探讨其改善腰肌衰老性萎缩的可能机制。方法采用随机数字表法将30只SPF级SD大鼠分为模型组、针刺组和非穴组,每组10只,于颈背部皮下注射D-半乳糖(300 mg/kg)制备亚急性腰肌衰老大鼠模型,另取10只作为空白组,颈背部皮下注射等量生理盐水。造模第9周开始干预,针刺组取双侧L3~L5“夹脊”针刺,非穴组取双侧非经非穴,每次30 min,每日1次,每周6次,连续4周。β半乳糖苷酶染色观察大鼠腰肌组织形态,TUNEL染色检测腰肌组织细胞凋亡情况,ELISA检测腰肌组织一氧化氮(NO)、内皮素-1(ET-1)含量,免疫荧光染色检测腰肌组织CD31表达和微血管密度,Western blot检测腰肌组织p-AMP活化的蛋白激酶(AMPK)、沉默信息调节因子1(SIRT1)、过氧化物酶体增殖物激活受体γ共激活因子1α(PGC-1α)蛋白表达。结果与空白组比较,模型组大鼠腰肌组织β-半乳糖苷酶染色阳性细胞明显增多,细胞凋亡率明显升高(P<0.001),腰肌组织NO含量明显降低(P<0.001),ET-1含量明显升高(P<0.001),CD31表达和微血管密度明显降低(P<0.001),p-AMPK、SIRT1、PGC-1α蛋白表达明显降低(P<0.01,P<0.001);与模型组比较,针刺组大鼠腰肌组织β-半乳糖苷酶染色阳性细胞明显减少,细胞凋亡率明显降低(P<0.001),NO含量明显升高(P<0.001),ET-1含量明显降低(P<0.01),CD31表达和微血管密度明显升高(P<0.01,P<0.001),p-AMPK、SIRT1、PGC-1α蛋白表达明显升高(P<0.05,P<0.01)。结论针刺“夹脊”可促进腰部骨骼肌血管生成,改善腰肌衰老性萎缩情况,其作用机制可能与激活AMPK/SIRT1/PGC-1α信号通路有关。
Objective To observe the effects of acupuncture at“Jiaji”on angiogenesis in rats with lumbar muscle aging based on AMPK/SIRT1/PGC-1α signaling pathway;To explore its possible mechanism of improving lumbar muscle aging atrophy.Methods Totally 30 SPF male SD rats were randomly divided into model group,acupuncture group and non-acupoint group,with 10 rats in each group.Subcutaneous injection of D-galactose(300 mg/kg)into the neck and back of rats was used to prepare a subacute lumbar muscle aging rat model.Another 10 rats were selected as the blank group,and an equal amount of normal saline was subcutaneously injected into the neck and back.Intervention began in the 9th week of modeling.The acupuncture group received acupuncture at bilateral L3-L5“Jiaji”,while the non-acupoint group received bilateral non-meridian and non-acupoint acupuncture,each time for 30 minutes,once a day,6 times a week,for 4 consecutive weeks.β-galactosidase staining was used to observe the morphology of rat lumbar muscle tissue;TUNEL staining was used to detect apoptosis of lumbar muscle tissue cells;ELISA was used to detect the contents of nitric oxide(NO)and endothelin-1(ET-1)in lumbar muscle tissue;immunofluorescence staining was used to detect CD31 expression and microvascular density in lumbar muscle tissue;Western blot was used to detect protein expressions of p-AMP activated protein kinase(AMPK),silencing information regulatory factor 1(SIRT1)and peroxisome proliferator activated receptor gamma co activator factor 1α(PGC-1α)in lumbar muscle tissue.Results Compared with the blank group,the positive cells ofβ-galactosidase staining in lumbar muscle tissue of the model group increased significantly,the apoptosis rate significantly increased(P<0.001),the NO content in lumbar muscle tissue significantly decreased(P<0.001),the ET-1 content significantly increased(P<0.001),the expression of CD31 and microvascular density significantly decreased(P<0.001),and the protein expressions of p-AMPK,SIRT1 and PGC-1αsignificantly decreased(P<0.01,P<0.001).Compared with the model group,the positive cells ofβ-galactosidase staining in lumbar muscle tissue of rats in the acupuncture group was significantly reduced,the apoptosis rate was significantly decreased(P<0.001),NO content significantly increased(P<0.001),ET-1 content was significantly reduced(P<0.01),CD31 expression and microvascular density significantly increased(P<0.01,P<0.001),and the protein expressions of p-AMPK,SIRT1,PGC-1αsignificantly increased(P<0.05,P<0.01).Conclusion Acupuncture at“Jiaji”can promote angiogenesis in lumbar skeletal muscle,and then improve the aging atrophy of lumbar muscle,and its mechanism may be related to the activation of AMPK/SIRT1/PGC-1αsignaling pathway.
作者
陈群
杨正瑜
汪冬梅
陈柏瑞
金京
陈少清
CHEN Qun;YANG Zhengyu;WANG Dongmei;CHEN Bairui;JIN Jing;CHEN Shaoqing(College of Adult Education,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;College of Rehabilitation Medicine,Fujian University of Tradional Chinese Medicine,Fuzhou 350122,China;School of Rehabilitation Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中国中医药信息杂志》
2025年第10期113-119,共7页
Chinese Journal of Information on Traditional Chinese Medicine
基金
福建省财政资助专项(X2021003)
福建中医药大学针灸学科开放课题(XZJ2022003)。
