摘要
为激发学生兴趣、培养适应产业发展需求的专业人才,重庆大学细胞基因工程实验课程团队基于转基因斑马鱼开展细胞基因工程研究,构建了直观可视化、病理现象化、动态实时化和手动操作化的转基因斑马鱼实验体系,首先构建表达硝基还原酶(NTR)和荧光蛋白的转基因鱼,然后用甲硝唑(MTZ)处理杀死荧光阳性细胞,直观可视地观察到转基因鱼脑部血管出血的病理现象。该实验体系包含细胞基因工程课程的所有核心实验,将该科研成果运用于课程教学,有效提高了本科课程的高阶性和创新性,也为本研一体化的细胞基因工程教学提供了新思路,提高了培养质量和培养效率。该实验课程的改革实践将为生物工程国家级一流本科专业的建设和生物医学工程一流学科建设赋能,为发展生物工程国家级一流本科专业注入动力。
To cultivate studentsinterest and develop professionals who can adapt to industry developments,building on our teams research in transgenic zebrafish genetic engineering,this course introduced an experimental system that features visual intuition,pathological phenomena,dynamic real-time observation,and hands-on operations.The transgenic fish expressing nitroreductase(NTR)and fluorescent proteins based on the metronidazole/nitroreductase(MTZ/NTR)cell ablation system was constructed.Treatment with MTZ eliminated fluorescent-positive cells,and pathological manifestations including brain hemorrhage,thereby generating visually intuitive results.This experiment covered all core experiments in the cell genetic engineering course.Applying these research findings to course teaching enhanced the advanced and innovative nature of undergraduate courses and provide new approaches for integrated undergraduate-graduate genetic engineering education,improving the quality and efficiency of training.This addressed the demands for experimental teaching innovation and practice.This experimental reform practice empowered the construction of double first-class disciplines,and the development of national first-class undergraduate disciplines in biomedical engineering and bioengineering,thereby advancing national first-class undergraduate programs in bioengineering.
作者
邱菊辉
蒲颖
雷明星
吴伟
王业启
张筱娟
王贵学
罗彦凤
QIU Juhui;PU Ying;LEI Mingxing;WU Wei;WANG Yeqi;ZHANG Xiaojuan;WANG Guixue;LUO Yanfeng(College of Bioengineering,Chongqing University,Chongqing 400030,China;Key Laboratory for Biorheological Science and Technology Chongqing University,Ministry of Education,Chongqing 400030,China;State and Local Joint Engineering Laboratory for Vascular Implants,Chongqing 400030,China)
出处
《生物学杂志》
北大核心
2025年第5期117-121,共5页
Journal of Biology
基金
重庆大学实验教学改革项目(2021S40)
重庆市高等教育教学改革研究重大项目(171003)
重庆大学生物工程一流专业核心课程群(2023)
国家自然科学基金青年科学基金项目(31701275)。
关键词
细胞基因工程实验教学
结果动态可视
斑马鱼
本研一体化
教学探索
experiment teaching of cellular genetic engineering
dynamic visualization of results
zebrafish
undergraduate and graduate integration course
teaching exploration
