摘要
目的探讨醛酮还原酶AKR1C3在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及与患者预后的关系。方法采用免疫组化染色检测86例OSCC肿瘤组织和癌旁组织中AKR1C3的表达,分析AKR1C3的表达与患者临床预后的相关性;应用Kaplan-Meier法对OSCC患者进行生存期分析,运用Cox回归模型对OSCC患者预后进行单因素和多因素分析,并与TCGA数据库结果比对验证。结果AKR1C3在OSCC肿瘤组织表达显著高于癌旁正常组织,肿瘤浸润前沿比肿瘤中心高表达,在肿瘤细胞和肿瘤相关成纤维细胞呈高表达,肿瘤相关炎性细胞无表达。χ^(2)检验比较OSCC患者中AKR1C3不同表达水平与临床病理特征的关系,结果显示:AKR1C3的高表达与较高肿瘤分期(P=0.001)、淋巴结转移(P=0.023)、肿瘤分化程度低(P=0.018)、最差浸润方式(P=0.024)及浸润深度(P=0.012)密切相关。采用Kaplan-Meier生存曲线分析:AKR1C3高表达与OSCC患者的总生存期(P=0.004)、无瘤生存期(P=0.019)以及无转移生存期(P=0.05)相关。Cox回归模型预后分析,单因素分析表明AKR1C3在肿瘤细胞中高表达是患者的肿瘤分期(P<0.001)、淋巴结转移(P<0.001)、肿瘤分化程度(P<0.001)、最差浸润方式(P<0.001)和浸润深度(P<0.001)的高风险因素,进一步多因素分析显示AKR1C3在肿瘤细胞中高表达是OSCC患者的独立预后因素(P=0.025)。结论AKR1C3表达与OSCC患者预后密切相关,OSCC中AKR1C3高表达患者的预后差,提示AKR1C3在OSCC的发生、发展中起重要作用。
Purpose To analyse the expression of aldo-keto reductase AKR1C3 in the tumour tissues of oral squamous carcinoma(OSCC)and the correlation of clinical prognosis of patients.Methods Immunohistochemical(IHC)staining was performed to analyze the expression characteristics of AKR1C3 in 86 cases of OSCC tumor tissues and paired paratumor normal tissue.The correlation between AKR1C3 expression and clinical prognosis was evaluated.Kaplan-Meier survival analysis was conducted to assess the survival outcomes of OSCC patients.Univariate and multivariate Cox regression analyses were employed to identify prognostic factors.The findings were further validated by comparison with the Cancer Genome Atlas(TCGA)database.Results The expression of AKR1C3 was significantly higher in OSCC tumor tissues than in paratumor normal tissue,with higher expression observed at the invasive front relative to the tumor centers.Immunohistochemical analysis revealed high expression in both tumor cells and tumor-associated fibroblasts,while no expression was detected in tumor-infiltrating immunocytes.Theχ^(2)test was used to compare the relationship between different expression levels of AKR1C3 and clinicopathological features in OSCC,the results showed that high expression of AKR1C3 was strongly associated with higher tumour stage(P=0.001),lymph node metastasis(P=0.023),differentiation(P=0.018),worst pattern of invasion(P=0.024)and depth of invasion(P=0.012).Kaplan-Meier survival curve analysis showed high expression of AKR1C3 was associated with overall survival(P=0.004),disease-free survival(P=0.019),and metastasis-free survival(P=0.05)in OSCC patients.Cox regression model was performed for prognostic analysis.Univariate analysis showed that high expression of AKR1C3 in tumour cells was a high-risk factor for patients’tumour stage(P<0.001),lymph node metastasis(P<0.001),differentiation(P<0.001),worst pattern of invasion(P<0.001),depth of invasion(P<0.001).Multivariate analysis further showed that high expression of AKR1C3 in tumour cells was an independent prognostic factor in OSCC patients(P=0.025).Conclusion The AKR1C3 expression is closely related to the prognosis of OSCC patients,and patients with high AKR1C3 expression have a poor prognosis,suggesting that AKR1C3 plays an important role in the occurrence and development of OSCC.
作者
周婷
张晓昕
张磊
金萬勇
泥艳红
胡勤刚
Zhou Ting;Zhang Xiaoxin;Zhang Lei;Jin Wanyong;Ni Yanhong;Hu Qingang(Department of Oral-Maxillofacial Surgery,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Institute of Stomatology,Nanjing University,Nanjing 210008,China;Central Laboratory of Stomatology,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Institute of Stomatology,Nanjing University,Nanjing 210008,China;Department of Oral Pathology,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Institute of Stomatology,Nanjing University,Nanjing 210008,China)
出处
《临床与实验病理学杂志》
北大核心
2025年第9期1200-1205,共6页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(82173159)
江苏省重点研发计划(BE2020628)。
关键词
口腔鳞状细胞癌
AKR1C3
临床病理特征
预后
oral squamous cell carcinomas
AKR1C3
clinicopathological characteristics
prognosis
