摘要
目的探讨急性睡眠剥夺(ASD)后对大鼠模型海马葡萄糖代谢和神经炎性反应的影响。方法将20只SD大鼠(雌雄各10只)通过随机抽样法分为4组(每组5只), 分别为雌性ASD组、雄性ASD组、雌性对照组、雄性对照组。其中ASD组构建ASD模型。72h睡眠剥夺后, 所有大鼠分2d分别进行^(18)F-FDG和N, N-二乙基-2-(2-(4-(2-^(18)F-氟乙氧基)苯基)-5, 7-二甲基吡唑并[1, 5-a]嘧啶-3-基)乙酰胺(^(18)F-DPA-714)microPET/CT脑显像, 比较大鼠海马^(18)F-FDG与^(18)F-DPA-714 SUV_(mean)的变化。另对大鼠进行脑组织病理学、免疫组织化学及免疫荧光染色检测。采用两独立样本t检验分析数据。结果 ^(18)F-FDG显像示雌雄ASD组和对照组海马SUV_(mean)(雌:4.11±0.35和1.89±0.28;雄:3.43±0.47和2.02±0.54)差异均具有统计学意义(t值:9.65、3.92, P值:<0.001、0.002)。^(18)F-DPA-714显像示雌雄ASD组和对照组海马SUV_(mean)(雌:0.28±0.01和0.28±0.02;雄:0.26±0.02和0.31±0.04)差异均无统计学意义(t值:-0.18、-2.24, P值:0.859、0.056)。相对分子质量18×103转位蛋白(TSPO)免疫组织化学显示雌雄ASD组和对照组在脑内海马区域的表达量(雌:0.19±0.02和0.19±0.01;雄:0.21±0.01和0.20±0.01)差异均无统计学意义(t值:-0.48、-1.67, P值:0.651、0.139)。免疫荧光染色示ASD 72h后脑内海马区域小胶质细胞胞体减小, 突起点及周围分支显著减少。结论 ASD 72 h后大鼠海马葡萄糖代谢增高, 但无明显的神经炎性反应。
Objective:To investigate the effects of acute sleep deprivation(ASD)on hippocampal glucose metabolism and neuroinflammation in rat models.Methods:Twenty SD rats(10 males and 10 females)were divided into four groups(five in each group)by random sampling method:female ASD group,male ASD group,female control group,and male control group.Among them,the ASD group constructed the ASD model.After 72h sleep deprivation,all rats underwent ^(18)F-FDG and N,N-diethyl-2-(2-(4-(2-^(18)F-fluoroethoxy)phenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl)acetamide(^(18)F-DPA-714)microPET/CT brain imaging in 2d to compare the changes of ^(18)F-FDG and ^(18)F-DPA-714 SUV_(mean) in the hippocampus of rats.Brain histopathology,immunohistochemistry and immunofluorescence staining were detected in rats.Independent-sample t test was used to analyze the data.Results:^(18)F-FDG imaging showed the hippocampal SUV_(mean) between ASD group and control group(female:4.11±0.35 vs 1.89±0.28;male:3.43±0.47 vs 2.02±0.54)were statistically significant(t values:9.65,3.92,P values:<0.001,0.002).^(18)F-DPA-714 imaging showed the hippocampal SUV_(mean) between ASD group and control group(females:0.28±0.01 vs 0.28±0.02;male:0.26±0.02 vs 0.31±0.04)were not statistically significant(t values:-0.18,-2.24,P values:0.859,0.056).The 18×103 translocator protein(TSPO)immunohistochemistry showed the expression in the hippocampal region of the brain between ASD group and control group(female:0.19±0.02 vs 0.19±0.01;male:0.21±0.01 vs 0.20±0.01)were not statistically different(t values:-0.48,-1.67,P values:0.651,0.139).Immunofluorescence staining showed that microglial cytosol in the hippocampal region of the brain decreased after 72h of ASD,and the protrusion points and surrounding branches were significantly reduced.Conclusion:Increased hippocampal glucose metabolism in rats is observed after 72 h of ASD without significant neuroinflammation.
作者
戴梦雅
相振宇
张妍
刘超锋
高洁
秦志星
王红亮
武志芳
李建国
李思进
Dai Mengya;Xiang Zhenyu;Zhang Yan;Liu Chaofeng;Gao Jie;Qin Zhixing;Wang Hongliang;Wu Zhifang;Li Jianguo;Li Sijin(Department of Nuclear Medicine,First Hospital of Shanxi Medical University,Shanxi Key Laboratory of Molecular Imaging,Collaborative Innovation Center for Molecular Imaging of Precision Medicine,Taiyuan 030001,China;Division of Radiation Medicine and Environmental Medicine,China Institute for Radiation Protection,Taiyuan 030006,China)
出处
《中华核医学与分子影像杂志》
北大核心
2025年第9期555-559,共5页
Chinese Journal of Nuclear Medicine and Molecular Imaging
基金
国家自然科学基金(U22A6008, 82027804)
山西省高等教育"百亿工程"专项资助(BYYX001)。
