摘要
目的探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)患者血清微小RNA(microRNA,miRNA)-335和miRNA-375表达及与患者病理特征和预后关系。方法采用病例对照研究方法,选择淮安市淮安医院于2021年3月至2023年3月收治的PTC患者94例作为PTC组,另选择同期健康体检者73例作为对照组。采用实时荧光定量PCR法测定血清miRNA-335和miRNA-375相对表达量。PTC患者随访至2024年9月30日,记录患者预后情况。比较两组、不同病理特征及不同预后患者血清miRNA-335和miRNA-375相对表达量。多因素logistic回归分析PTC患者预后的独立危险因素。结果PTC组血清miRNA-335相对表达量(2.35±0.68)高于对照组(0.98±0.04),而miRNA-375相对表达量(0.65±0.21)低于对照组(1.01±0.02,均P<0.001)。PTC患者中,不同肿瘤直径、肿瘤位置、肿瘤数目和脉管侵犯情况血清miRNA-335和miRNA-375相对表达量比较,差异均无统计学意义(均P>0.05);TNM分期Ⅲ~Ⅳ期血清miRNA-335相对表达量高于Ⅰ~Ⅱ期,而miRNA-375相对表达量低于Ⅰ~Ⅱ期(均P<0.001);有淋巴结转移者血清miRNA-335相对表达量高于无淋巴结转移者,而miRNA-375相对表达量低于无淋巴结转移者(均P<0.001)。预后不良组血清miRNA-335相对表达量高于预后良好组,而miRNA-375相对表达量低于预后良好组(均P<0.001)。脉管侵犯、淋巴结转移、miRNA-335高表达和miRNA-375低表达均为PTC患者预后的独立危险因素(均P<0.05)。结论PTC患者血清miRNA-335高表达而miRNA-375低表达,且miRNA-335和miRNA-375表达与TNM分期、淋巴结转移及预后密切相关,值得临床关注。
Objective To study the expression of serum microRNA(miRNA)-335 and miRNA-375 in patients with papillary thyroid carcinoma(PTC)and their relationship with pathological features and prognosis.Methods Using a case-control study method,94 PTC patients admitted to Huaian Hospital of Huaian City from March 2021 to March 2023 were selected as PTC group.Another 73 healthy individuals who underwent physical examinations during the same period were selected as control group.Quantitative real-time PCR was used to determine the relative expression levels of serum miRNA-335 and miRNA-375.PTC patients were followed up until September 30,2024,and the patient's prognosis was record.The relative expression levels of serum miRNA-335 and miRNA-375 were compared between the two groups,among different pathological features,and among patients with different prognoses.Multivariate logistic regression analysis was performed to identify independent risk factors for prognosis in PTC patients.Results The relative expression level of serum miRNA-335 in the PTC group(2.35±0.68)was higher than that in the control group(0.98±0.04),while the relative expression level of miRNA-375(0.65±0.21)was lower than that in the control group(1.01±0.02,P<0.001).There was no statistically significant differences in the relative expression levels of serum miRNA-335 and miRNA-375 among PTC patients with different tumor diameters,tumor locations,tumor numbers,and vascular invasion(P>0.05).The relative expression level of serum miRNA-335 in TNM stagesⅢ-Ⅳwas higher than that in stagesⅠ-Ⅱ,while the relative expression level of miRNA-375 in TNM stagesⅢ-Ⅳwas lower than that in stagesⅠ-Ⅱ(P<0.001).The relative expression level of serum miRNA-335 of patients with lymph node metastasis was higher than that of patients without lymph node metastasis,while the relative expression level of serum miRNA-375 of patients with lymph node metastasis was lower than that of patients without lymph node metastasis(P<0.001).The relative expression level of serum miRNA-335 in the poor prognosis group was higher than that in the good prognosis group,while the relative expression level of miRNA-375 in the poor prognosis group was lower than that in the good prognosis group(P<0.001).Vascular invasion,lymph node metastasis,high expression of miRNA-335,and low expression of miRNA-375 were all independent risk factors for prognosis in PTC patients(P<0.05).Conclusion Patients with PTC have high serum miRNA-335 expression and low miRNA-375 expression,and the expression of miRNA-335 and miRNA-375 is closely related to TNM staging,lymph node metastasis,and prognosis,which deserves clinical attention.
作者
池浩
樊卫
李红阳
王坦
王维
杭程
Chi Hao;Fan Wei;Li Hongyang;Wang Tan;Wang Wei;Hang Cheng(Department of Laboratory Medicine,Huai'an Clinical Medical College of Jiangsu University,Huaian Hospital of Huaian City,Huai'an 223200,China;Department of Oncology,Lianshui People's Hospital Affiliated to Kangda College of Nanjing Medical University,Huai'an 223400,China;Department of General Surgery,Lianshui People's Hospital Affiliated to Kangda College of Nanjing Medical University,Huai'an 223400,China;Department of Clinical Laboratory,Lianshui People's Hospital Affiliated to Kangda College of Nanjing Medical University,Huai'an 223400,China;Department of Pathology,Huai'an No.3 People's Hospital,Huai'an 223200,China)
出处
《中华地方病学杂志》
北大核心
2025年第7期530-534,共5页
Chinese Journal of Endemiology
基金
江苏省卫生健康委科研项目(Z2021044A)。
