摘要
为探究酸性土壤中锰毒胁迫对不同油菜品种影响及油菜耐锰毒机制,以41份甘蓝型油菜品种为材料,通过营养液培养试验分析其在正常锰和锰毒害条件下的表型,计算锰毒处理下的总鲜质量与正常处理下的总鲜质量的比值,鉴定出锰毒耐受品种(manganese toxin tolerant variety,Mn-T)427和敏感品种(manganese toxin sensitive variety,Mn-S)907,并初步解析油菜耐锰毒的生理机制。结果显示,锰毒胁迫显著抑制敏感品种907生长,其地上部鲜质量和光合速率显著下降,叶绿素降解严重,根系生长受到抑制;同时,敏感品种根中活性氧过量积累,表明其抗氧化系统清除效率低下。相比之下,耐受品种427表现出显著优势,其生物量受限小,能通过增加根长和根表面积等优化根系构型以适应锰毒胁迫,叶绿素损失较小,光合作用受锰毒影响小,并具备更强的抗氧化能力。综上,与锰毒敏感品种相比,锰毒耐受品种通过调控根系生长、维持光合作用及增强抗氧化系统以缓解锰毒对其的损伤。
To explore the effects of manganese toxicity stress on different rapeseed varieties and the mechanisms of manganese tolerance in rapeseed under acidic soil conditions,41 varieties of Brassica napus were used to analyze their phenotypes under normal manganese and manganese toxicity conditions with nutrient solution(300μmol/L MnCl2)cultivation to study the effects of manganese toxicity in acidic soil on different varieties of B.napus and the mechanisms of manganese toxicity tolerance in B.napus.The ratio of total fresh mass under manganese toxicity treatment to total fresh mass under normal treatment was calculated.The manganese toxin tolerant variety 427(Mn-T 427)and the manganese toxin sensitive variety 907(Mn-S 907)were identified.The physiological mechanism of manganese tolerance in B.napus was preliminarily analyzed.The results showed that manganese toxicity stress significantly inhibited the growth of Mn-S 907,resulting in a significant decrease in shroot fresh weight and photosynthetic rate,severe degradation of chlorophyll,and inhibition of root growth.The excessive accumulation of reactive oxygen species(ROS)in the roots of Mn-S 907 indicated a low clearance efficiency of their antioxidant system.In contrast,Mn-T 427 had significant advantages including less limitation of biomass and the ability to optimize root architecture by increasing root length and surface area to adapt to manganese toxicity stress.The loss of chlorophyll was smaller,photosynthesis was less affected by manganese toxicity,and it had stronger antioxidant capacity.Compared with Mn-S 907,Mn-T 427 alleviates the damage caused by manganese toxicity through regulating root growth,maintaining photosynthesis,and enhancing antioxidant systems.It will provide important germplasm resources and a theoretical foundation for breeding B.napus with high yield in acidic soils.
作者
张雯
张鹏超
易伯涛
孙明珠
戴志刚
谢敏
王创
徐芳森
丁广大
ZHANG Wen;ZHANG Pengchao;YI Botao;SUN Mingzhu;DAI Zhigang;XIE Min;WANG Chuang;XU Fangsen;DING Guangda(College of Resources and Environment/Microelement Research Center/Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River),Ministry of Agriculture and Rural Affairs,Huazhong Agricultural University,Wuhan 430070,China;Jiangxi Agricultural Technology Extension Center,Nanchang 330046,China;Hubei Cultivated Land Quality and Fertilizer Station,Wuhan 430070,China;Yuanzhou District Agriculture and Rural Affairs Bureau of Yichun City,Jiangxi Province,Yichun 336099,China)
出处
《华中农业大学学报》
北大核心
2025年第5期80-87,共8页
Journal of Huazhong Agricultural University
基金
国家重点研发计划项目(2022YFD1900705)。
关键词
油菜
锰毒
极端品种
根系
光合作用
抗氧化系统
Brassica napus L.
manganese toxicity
extreme varieties
root
photosynthesis
antioxidant systems
