摘要
为解析微塑料对中药材黄芩(Scutellaria baicalensis)植株的生长影响及其毒理学响应,通过营养液培养的方式,检测了添加微塑料浓度为500 mg·L^(-1)、粒径为10μm左右的聚苯乙烯微塑料(PS-MPs)、聚乙烯微塑料(PE-MPs)及聚丙烯微塑料(PP-MPs)营养液对黄芩植株生长状况、抗氧化酶活性的影响,并进一步探究了不同类型微塑料对黄芩植株的毒性作用。结果表明:PE-MPs对黄芩生长的影响相对较小,而PS-MPs和PP-MPs的影响更为显著。PS-MPs处理导致光合色素含量降低。抗氧化酶活性方面,PS-MPs和PE-MPs处理导致酶活性显著提升,PP-MPs处理影响较小。代谢层面上,PS-MPs和PP-MPs影响显著,主要影响半乳糖代谢、甘氨酸、丝氨酸和苏氨酸代谢等途径,而PE-MPs主要影响糖代谢和TCA循环。此外,PP-MPs对黄芩根中汉黄芩素和黄芩素的影响最为显著,对黄芩苷和汉黄芩苷的影响相对较小。研究结果表明,不同类型微塑料对黄芩生长和代谢的影响存在差异,其中PS-MPs和PP-MPs主要影响黄芩植株的生长,而PE-MPs的影响比较小。PE-MPs主要影响黄芩植株的糖代谢和TCA循环,而PS-MPs和PP-MPs则主要影响TCA循环、半乳糖代谢、甘氨酸、丝氨酸和苏氨酸代谢,并且对黄芩植株的主要化合物的含量也产生一定的影响。
To analyze the effects of microplastics on the growth of Scutellaria baicalensis plants,a traditional Chinese medicine,and their toxicological responses.The effects of adding microplastics of polystyrene microplastics(PS-MPs),polyethylene microplastics(PE-MPs)and polypropylene microplastics(PP-MPs)with a concentration of 500 mg·L^(-1)and a particle size of about 10μm on the growth and antioxidant enzyme activities of S.baicalensis plants were examined in nutrient solution cultures and the toxic effects of the different microplastics on the S.baicalensis plants were further investigated.The toxic effects of different types of microplastics on S.baicalensis plants were further investigated.The results showed that the effect of PE-MPs on the growth of S.baicalensis was relatively small,while the effects of PS-MPs and PP-MPs were more significant.In terms of photosynthetic pigment content,PS-MPs led to a significant decrease.At the antioxidant enzyme activity level,PS-MPs and PE-MPs treatments significantly increased,while PP-MPs treatment had less effect.At the metabolic level,PS-MPs and PP-MPs microplastics had significant effects,mainly affecting the pathways of galactose metabolism,glycine,serine,and threonine metabolism,whereas PE-MPs mainly affected sugar metabolism and TCA cycle.In addition,PP-MPs had the most significant effect on baicalein and baicalein in S.baicalensis roots,and relatively little effect on baicalein and baicalin.The results indicate that the impacts of various types of microplastics on the growth and metabolism of S.baicalensis differ,with PS-MPs and PP-MPs primarily influencing the growth of S.baicalensis plants,while PE-MPs exert a relatively minor effect.PE-MPs predominantly impact the sugar metabolism and the TCA cycle in S.baicalensis plants,while PS-MPs and PP-MPs predominantly affect the TCA cycle,galactose metabolism,as well as the metabolism of glycine,serine,and threonine.Additionally,they influence the content of major compounds within S.baicalensis plants.
作者
高雅楠
王论言
李欣雨
贾向前
张彦龙
刘洋
GAO Yanan;WANG Lunyan;LI Xinyu;JIA Xiangqian;ZHANG Yanlong;LIU Yang(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education&Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region&Key Laboratory of Microbiology,College of Heilongjiang Province&School of Life Sciences,Heilongjiang University,Harbin 150080,China;Post-Doctoral Scientific Research Workstation of Heilongjiang Boli Economic Development Zone Management Committee,Boli 154500,China)
出处
《农业环境科学学报》
北大核心
2025年第8期2120-2133,共14页
Journal of Agro-Environment Science
基金
黑龙江省自然科学基金(YQ2023C037)
国家自然科学基金青年科学基金(32301299)。
关键词
微塑料
黄芩
生理响应
氧化应激
代谢产物
microplastics
Scutellaria baicalensis
physiological response
oxidative stress
metabolites
