摘要
本试验旨在研究不同蛋白质水平饲粮中添加胍基乙酸(GAA)对牦牛血浆激素指标、血清抗氧化指标、肉品质及抗氧化能力的影响,为牦牛养殖合理使用GAA提供技术参考。选取48头4~5岁公牦牛,随机分为6组,每组8个重复,每个重复1头牛。采用2×3双因素试验设计,2个饲粮蛋白质水平分别为12.88%(低)和17.00%(高),3个GAA添加水平分别为精料的0、0.1%、0.2%(干物质基础)。预试期10 d,正试期90 d。结果表明:1)低蛋白质饲粮组血浆胰岛素(INS)和胰岛素样生长因子-1(IGF-1)含量显著低于高蛋白质饲粮组(P<0.05)。0.2%GAA组血浆INS含量显著低于0和0.1%GAA组(P<0.05)。2)低蛋白质饲粮组血清总抗氧化能力(T-AOC)显著高于高蛋白质饲粮组(P<0.05)。3)低蛋白质饲粮组背最长肌pH及赖氨酸、苏氨酸、缬氨酸、异亮氨酸、丙氨酸、谷氨酸、丝氨酸、天门冬氨酸含量显著高于高蛋白质饲粮组(P<0.05),背最长肌苯丙氨酸、脯氨酸、胱氨酸、酪氨酸含量显著低于高蛋白质饲粮组(P<0.05)。0.1%GAA组背最长肌胱氨酸含量显著低于0和0.2%GAA组(P<0.05)。4)0.2%GAA组背最长肌谷胱甘肽过氧化物酶(GSH-Px)活性显著低于0和0.1%GAA组(P<0.05)。由此可见,在低蛋白质饲粮中添加0.2%精料比例的GAA时,牦牛血浆激素指标、血清抗氧化指标、肉品质、肌肉氨基酸含量及肌肉抗氧化指标的动态平衡达到最优,适用于牦牛养殖。
This experiment was conducted to investigate the effects of guanidino acetic acid(GAA)supplementation in diets with different protein levels on plasma hormone indices,serum biochemical indices,meat quality and antioxidant capacity of yaks(Bos grunniens),and to provide technical reference for the rational use of GAA in yak breeding.Forty-eight male yaks aged 4 to 5 years were selected and randomly divided into 6 groups with 8 replicates per group and 1 yak per replicate.Using a 2×3 two-factor experimental design,the 2 dietary protein levels were 12.88%(low)and 17.00%(high),respectively,and the 3 GAA supplemental levels were 0,0.1%,0.2%of the concentrate(dry matter basis),respectively.The pre-experimental period lasted for 10 days,and the experimental period lasted for 90 days.The results showed as follows:1)the plasma insulin(INS)and insulin-like growth factor-1(IGF-1)contents of low protein diet group were significantly lower than those of high protein diet group(P<0.05).The plasma INS content of 0.2%GAA group was significantly lower than that of 0 and 0.1%GAA groups(P<0.05).2)The serum total antioxidant capacity(T-AOC)of low protein diet group was significantly higher than that of high protein diet group(P<0.05).3)The pH and contents of lysine,threonine,valine,isoleucine,alanine,glutamic acid,serine and aspartic acid in Longissimus dorsi of low protein diet group were significantly higher than those of high protein diet group(P<0.05),and the contents of phenylalanine,proline,cystine,and tyrosine in Longissimus dorsi were significantly lower than those of high protein diet group(P<0.05).The Longissimus dorsi cystine content of 0.1%GAA group was significantly lower than that of 0 and 0.2%GAA groups(P<0.05).4)The Longissimus dorsi glutathione peroxidase(GSH-Px)activity of 0.2%GAA group was significantly lower than that of 0 and 0.1%GAA groups(P<0.05).It is concluded that when 0.2%GAA of the concentrate is added to the low protein diet,the dynamic balance of plasma hormone indices,serum biochemical indices,meat quality,muscle amino acids contents and muscle antioxidant indices is optimised,which is suitable for yak breeding.
作者
姜菲
游茵洁
顾以韧
宋和键
吴建平
黄艳玲
JIANG Fei;YOU Yinjie;GU Yiren;SONG Hejian;WU Jianping;HUANG Yanling(Key Laboratory of Animal Science of National Ethnic Affairs Commission,Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resources Reservation and Utilization,College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Bureau of Agriculture and Rural Affairs of Guangyuan City,Guangyuan 628000,China;The Agriculture and Rural Affairs Bureau of Aba Tibetan and Qiang Autonomous Prefecture,Aba 624099,China;Sichuan Ganzi Prefecture Animal Science Research Institute,Kangding 626000,China)
出处
《动物营养学报》
北大核心
2025年第9期6032-6046,共15页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
牦牛高效养殖关键技术与集成应用研究(280130033501)
四川省肉牛创新团队建设项目(sccxtd-2023-13)
高原畜禽营养与饲料资源评价与利用(2024CXTD17)。
关键词
胍基乙酸
牦牛
激素
抗氧化
肉品质
guanidino acetic acid
yaks
hormone
antioxidant
meat quality
