摘要
建立了超声辅助分散液液微萃取-高效液相色谱法对乳品中雌二醇含量进行快速、高灵敏测定的分析方法。考察了萃取剂种类及用量、分散剂种类及用量四个因素对雌二醇萃取效果的影响。得到的最佳微萃取条件为:200μL三氯甲烷作为萃取剂,300μL乙腈作为分散剂。在最佳萃取条件下,方法的线性范围为0.025~2.0μg/mL,检出限0.008μg/mL,相对标准偏差(RSD)小于5.1%,加标回收率在78.1%~92.0%之间。结果表明,该方法简单快速、环境友好、灵敏度高,可成功应用于乳品中雌二醇含量的测定。
A rapid and highly sensitive analysis method was established for the determination of estradiol in dairy products using ultrasound assisted liquid-liquid microextraction coupled with high-performance liquid chromatography.Effects of the types,the volumes of the extraction solvents and the disperser solvents on the extraction efficiency of estradiol were investigated.The optimal microextraction conditions were obtained as follows:200μL chloroform as the extractant and 300μL acetonitrile as the dispersant.Under the optimal extraction conditions,the linear range of estradiol was from 0.025 to 2.0μg/mL,with a detection limit of 0.008μg/mL.The relative standard deviation(RSD)was less than 5.1%,and the recovery in spiked samples was between 78.1%and 92.0%.The results indicated that the method was simple,fast,environmentally friendly,highly sensitive,and can be successfully applied to the determination of estradiol in dairy products.
作者
张朝辉
覃淋
李丹
文星星
姜放军
黄嘉苗
ZHANG Zhaohui;QIN Lin;LI Dan;WEN Xingxing;JIANG Fangjun;HUANG Jiamiao(Hunan Biological and Electromechanical Polytechnic,Hunan Changsha 410127,China)
出处
《广州化工》
2025年第18期105-108,共4页
GuangZhou Chemical Industry
关键词
分散液液微萃取
高效液相色谱
乳品
雌二醇
dispersive liquid-liquid microextraction
high performance liquid chromatography
dairy
estradiol
