摘要
【目的】为探究不同翅型荻草谷网蚜(Sitobion miscanthi)体内微生物群落组成及多样性。【方法】利用Illumina NovaSeq平台对荻草谷网蚜有翅/无翅四龄若蚜和成蚜体内的细菌16S rRNA基因V3-V4区及真菌ITS1区进行高通量测序。【结果】共获得737705条细菌优质序列和763626条真菌优质序列,聚类得到5284个细菌OTUs和709个真菌OTUs。细菌优势菌门为厚壁菌门(Firmicutes)和变形菌门(Proteobacteria),优势菌属为布赫纳氏菌属(Buchnera)、葡萄球菌属(Staphylococcus)和不动杆菌属(Acinetobacter);真菌优势菌门为子囊菌门(Ascomycota)和担子菌门(Basidiomycota),优势属为Cyberlindnera。分析发现,与无翅四龄若蚜相比,有翅四龄若蚜体内的厚壁菌门丰度高,葡萄球菌属丰度高,布赫纳氏菌属丰度低;而有翅和无翅成蚜间各细菌门/属的丰度无显著差异。真菌群落中,有翅成蚜体内的子囊菌门和Cyberlindnera属丰度显著低于无翅成蚜,但四龄若蚜翅型间无显著差异。【结论】共生菌群落结构与荻草谷网蚜翅型分化密切相关,为解析其翅型调控机制及开发基于共生菌的绿色防控技术提供了理论依据。
[Objective]This study aimed to explore the community structure and microbial diversity in winged and wingless Sitobion miscanthi.[Method]The study employed Illumina NovaSeq sequencing of the bacterial 16S rRNA gene(V3-V4 region)and fungal ITS1 region to investigate the differences in symbiotic microbial communities between winged and wingless fourth-instar nymphs and adults.[Result]A total of 737705 high-quality bacterial sequences and 763626 fungal sequences were obtained,clustering into a total of 5284 bacterial OTUs and 709 fungal OTUs.The dominant bacterial phyla were Firmicutes and Proteobacteria,with Buchnera,Staphylococcus,and Acinetobacter as the predominant genera.As for fungi,Ascomycota and Basidiomycota were the dominant phyla,with Cyberlindnera as the most abundant genus.Comparative analysis revealed that in the bacterial community,winged fourth-instar nymphs exhibited significantly higher Firmicutes abundance,reduced Buchnera abundance,and increased Staphylococcus abundance compared to that of wingless nymphs,while no significant differences were observed between winged and wingless adults.In the fungal community,winged adults showed significantly lower Ascomycota and Cyberlindnera abundance than that of wingless adults,but no significant differences were found in fourth-instar nymphs.[Conclusion]These findings suggest that symbiotic microbiota may play a role in the wing dimorphism of S.miscanthi,providing a theoretical basis for elucidating the regulatory mechanisms of wing differentiation and developing novel symbiotic microbe-based pest control strategies.
作者
顾照华
李玉洁
徐昭焕
GU Zhaohua;LI Yujie;XU Zhaohuan(School of Agronomy Sciences,JiangxiAgricultural University,Nanchang 330045,China)
出处
《生物灾害科学》
2025年第3期340-347,共8页
Biological Disaster Science
基金
国家自然科学基金项目(31760513)。
关键词
荻草谷网蚜
翅型分化
微生物多样性
高通量测序
Sitobion miscanthi
wing differentiation
microbial diversity
high-throughput sequencing
