摘要
【目的】从分子水平分析马铃薯野生种、地方种的遗传多样性并建立马铃薯种质资源的指纹图谱库。【方法】利用简单串接重复序列(simple sequence repeat,SSR)分子标记技术,对国际马铃薯中心亚太中心保存的73份马铃薯野生种、地方种的遗传多样性进行研究。【结果】平均等位基因数(Na)为1.8437,Nei’s平均基因多样性指数为0.0659,平均Shannon信息指数为0.1286,多态性信息含量PIC平均值为0.8484,表明供试的73份马铃薯种质资源多态性高但遗传差异小。Structure群体结构将73份马铃薯种质分为7个类群,UPGMA聚类分析分为4个类群。【结论】从14对引物中筛选出可完全区分73份种质的5对核心引物,构建了73份马铃薯野生种和地方种的指纹图谱,为后续马铃薯种质鉴定和筛选体系的研究提供了理论基础和技术支撑。
【Objective】Analyze genetic diversity of wild and landrace potato species at the molecular level and establish a fingerprint database for potato germplasm resources.【Method】SSR(Simple Sequence Repeat)molecular markers was used to analyze the genetic diversity of 73 wild potato and landrace potato species preserved at the CIP-China Center for Asia Pacific.【Result】The genetic diversity analysis revealed an average number of alleles(Na)of 1.8437,Nei’s gene diversity index of 0.0659,Shannon’s information index of 0.1286,and an average polymorphism information content(PIC)value of 0.8484,indicating high polymorphism but low genetic differentiation among the 73 germplasm resources.The germplasms were classified into seven subpopulations by Structure analysis,whereas they were divided into four groups via UPGMA clustering.【Conclusion】Five pairs of core primers are selected from 14 SSR primers,enabling complete differentiation of all 73 accessions.These primers were used to construct unique DNA fingerprints for the 73 wild and landrace potato accessions.This work provides a theoretical foundation and technical framework for future germplasm identification and screening systems in potato breeding.
作者
卢瑶
袁平平
金鑫
毛向红
范向斌
白小东
LU Yao;YUAN Ping-ping;JIN Xin;MAO Xiang-hong;FAN Xiang-bin;BAI Xiao-dong(Shanxi Agricultural University,High Latitude Crops Institute to Shanxi Academy,Shanxi Provincial Key Laboratory of Potato Genetic Improvement and Germplasm Innovation,Datong 037006;CIP-China Center for Asia Pacific,Beijing 102100;Ningxia Hui Autonomous Region Raw Seed Farm,Yinchuan 750200)
出处
《生物技术通报》
北大核心
2025年第9期94-104,共11页
Biotechnology Bulletin
基金
山西省高等学校科技创新项目(2023L038)
山西农业大学高寒区作物研究所所级项目(GHS202402)
国家马铃薯产业技术体系(CARS-09-ES02)
山西省现代农业产业技术体系建设专项资金资助(2025CYJSTX06)
大同市应用基础研究计划项目(2024078)。
关键词
马铃薯
野生种
地方种
遗传多样性
SSR
指纹图谱
potato
wild potato
landraces
genetic diversity
SSR(Simple Sequence Repeat)
fingerprinting
