摘要
本研究利用RNA-seq测序技术对洛河水系黄河雅罗鱼肌肉进行转录组测序与分析。结果共获得了90,078,144条Raw Reads(原始序列数据),总碱基数为13.95 G。经数据拼装后,最终获得了125,466条Unigenes(单基因组),其长度范围在177~95,389 bp,平均长度为534 bp。这些Unigenes在六大数据库(Nr、Swiss-Prot、Pfam、NOG、GO和KEGG)都能同时被成功注释的有43,681条。GO功能结果显示:DNA模板转录的调控、细胞膜和金属离子结合注释较多。KEGG结果显示:MAPK信号通路、Wnt信号通路和细胞自噬富集较多。这些研究结果的获得可完善洛河水系黄河雅罗鱼转录组的注释信息,也为后续进行洛河水系黄河雅罗鱼肌肉生长与发育的分子调控机制研究提供数据支撑。
In this study,RNA-seq technology was used to conduct a transcriptome analysis on the muscle tissues of Leuciscus chuanchicus in Luohe River System.The results yielded 90,078,144 raw reads,with a total nucleotides of 13.95 G.After data assembly,125,466 Unigenes were ultimately obtained,with lengths ranging from 177 to 95,389 bp and an average length of 534 bp.Among these Unigenes,43,681 were successfully annotated in all six databases(Nr,Swiss-Prot,Pfam,NOG,GO and KEGG).GO function results show that the regulation of DNA template transcription,cell membrane and metal ion binding are more annotated.The KEGG analysis showed that the MAPK signaling pathway,Wnt signaling pathway,and autophagy were the more enriched.These findings not only improve the annotation information of the transcriptome of Leuciscus chuanchicus in Luohe River System but also provide a data foundation for subsequent research on the molecular regulatory mechanisms underlying muscle growth and development in this species.
作者
刘亚娟
孙晓辉
朱文文
赵道全
黄勇
Liu Yajuan;Sun Xiaohui;Zhu Wenwen;Zhao Daoquan;Huang Yong(Luoyang City Agricultural Technology Extension Service Center,Luoyang,471000,China;Food and Drug college of Luoyang Vocational and Technology College,Luoyang 471004,China;Henan Academy of Fishery Sciences,Zhengzhou 450044,Henan;College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,471000,China)
出处
《河南水产》
2025年第3期13-15,19,共4页
HENAN FISHERIES
基金
河南高等学校重点科研项目(No.23A240003)。
