摘要
【目的】明确蕨麻与病原菌互作机制、差异代谢通路和寄主植物病程相关的关键基因筛选等,为蕨麻根腐病防治提供理论依据。【方法】以前期分离筛选出的蕨麻致病真菌代表菌株对健康蕨麻进行侵染,利用转录组学对其侵染及互作进行分析。【结果】从燕麦镰刀菌侵染蕨麻根系中筛选获得14737个蕨麻响应镰刀菌根腐病致病性侵染的差异基因,其中12547个上调表达,2190个下调表达。差异基因功能富集分析表明,蕨麻感病株与健康株间存在明显差异,GO富集结果显示上调基因主要富集在DNA模板转录、水解酶活性和DASH综合体,下调基因主要富集在基于维管运动、水解O-糖基化合物的水解酶活性和外质体;KEGG富集结果显示所有差异基因主要集中于代谢和遗传信息处理这两类分支。对差异表达关键基因进行筛选可知,与诸如细胞壁加固与降解、抗氧化胁迫、抗病信号转导、毒素产生、植物激素信号转导等重要功能相关的关键基因均出现差异表达,且表达模式不一。利用qRT-PCR技术对筛选出的10个关键差异表达基因进行验证,其基因表达量及趋势与转录组测序结果一致。【结论】蕨麻响应根腐病致病真菌侵染的主要途径可能为:受到燕麦镰刀菌侵染的蕨麻块根内萜类次生代谢加强;诱导水杨酸等防御激素的表达如植保素、过氧化物酶类、萜类化合物等次生代谢产物被诱导;可能存在真菌被植物模式识别受体(PRR)感知而触发植物免疫应答。
[Objective]The paper aimed to clarify the interaction mechanisms between Potentila anserina L.and pathogenic bacteria,differential metabolic pathways and key gene screening related to the disease course of the host plant,etc.,so as to provide a theoretical basis for the control of P.anserina L.root rot disease.[Method]A representative strain of pathogenic fungi of P.anserina L.,which was isolated and screened in the previous stage,was employed to inoculate healthy P.anserina L.plants.Subsequently,transcriptomic analysis was utilized to explore the process of infestation and the interactions between the pathogen and the host.[Result]14737 differential genes of P.anserina L.responding to Fusarium root rot pathogenic infestation were screened and obtained from Fusarium ratio-infested root system of P.anserina L.,in which 12547 were up-regulated and 2190 down-regulated expression.Functional enrichment analysis of dfferential expression of genes showed significant differences between the susceptible and healthy P.anserina L.strains.GO enrichment results showed that up-regulated genes were mainly enriched in DNA template transcription,hydrolase activity and DASH complex.In contrast,down-regulated genes were mainly enriched in hydrolase activity based on vascular movement and hydrolyzed O-glycosyl compounds and extracellular bodies.KEGG enrichment results showed that all dfferential expression of genes were mainly concentrated in the metabolism and genetic information processing branches.Key genes related to cell wall reinforcement and degradation,resistance to oxidative stress,disease resistance signal transduction,toxin production,plant hormone signal transduction,and other important functions exhibited dfferentially expressed,with which gene presenting unique expression patterns.The qRT-PCR technique was utilized to validate the 10 key differentially expressed genes that were screened,and their gene expressions and trends were consistent with the transcriptome sequencing results.[Conclusion]The principal response pathways of P.anserina L.to the infestation by root rot-causing fungi may be as follows:An enhancement of terpenoid secondary metabolism within Fusarium oatmeal-infected roots;The induction of the expression of defense associated hormones such as salicylic acid;The induction of secondary metabolites such as phytochelatins,peroxisomes and terpenoids;And the possible presence of a fungus that is triggered by a plant immune response by plant pattern recognition receptor(PRR)sensing.
作者
吕博文
李军乔
李晨芹
LYU Bo-wen;LI Jun-qiao;LI Chen-qin(College of Ecological Environment and Resources,Qinghai Nationalities University,Xining 810007,China;Key Laboratory of High Value Utilization of Characteristic Economic Plants of Qinghai Province,Xining 810007,China;Tibetan Plateau Potentlla anserina L.Research Centre,Qinghai Nationalities University,Xining 810007,China)
出处
《西南农业学报》
北大核心
2025年第6期1237-1255,共19页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(U23A20152)。
关键词
蕨麻
根腐病
侵染
转录组学
发病机理
Potentlla anserina L.
Root rot
Infect
Transcriptome analysis
Pathogenesis
