摘要
Background:Ultrashort wave(USW)therapy has been reported to alleviate cerebral ischemia/reperfusion(IR)injury,however the underlying mechanisms remain elusive.The aim of this study was to observe the effect of non-thermal USW therapy on neuronal damage and expression of heat shock protein 70(HSP70)after cerebral IR in rats.Methods:Focal ischemia-reperfusion(IR)was induced in SpragueeDawley rats by middle cerebral artery occlusion/reperfusion(MCAO/R).The Ninety-two rats(both male and female)were screened using the Zea-Longa 5 grade evaluation.Included rats were then randomly divided into blank,sham,model 1-day,model 3-day,model 7-day,USW 1-day,USW 3-day,or USW 7-day groups.All rats in the model groups received sham USW treatment,while rats in the USW groups received USWtreatment,for 1,3,or 7 days.We assessed the National Institutes of Health Stroke Scale,brain infarction volumes,ultrastructural damage scores using electron microscopy,and HSP70 expression by western blotting between the different groups.Results:USW treatment reduced the National Institutes of Health Stroke Scale,infarction volume,and ultrastructural neuronal damage,and increased expression of HSP70,in the hippocampal CA1 region.Conclusions:Non-thermal USW therapy may improve neurological function,decrease infarction volume,and reduce neuronal damage by increasing HSP70 expression following cerebral IR injury.
基金
granted by the National Natural Science Foundation of China(No.81971098).
