摘要
为了解嗜水气单胞菌(Aeromonas hydrophila)毒力因子分布及其耐药表型状况,采集102份动物样本和15份水源环境样本,分离培养A.hydrophila并进行鉴定,随后应用PCR方法测定细菌的ear、alt、hly、lip、act 5个毒力基因,并采用Kirby-Bauer纸片扩散法测定A.hydrophila对头孢噻肟、头孢曲松、氧氟沙星等10种药物的敏感性。结果显示,从117份样本中共分离得到116株鱼源A.hydrophila、43株蛙源A.hydrophila、11株蛇源A.hydrophila、16株水源A.hydrophila;毒力基因act的阳性检出率最高(73.65%),其次为ear(65.59%),最常见毒力基因型为“ear+hly+act”,且75.81%的A.hydrophila至少携带3种及以上毒力基因;186株A.hydrophila均对2种及以上抗生素耐药,多重耐药率为100%,MAR index在0.2~0.9,“AMX+AMP+VA+GEN”耐药型最为常见;耐药表型及毒力基因型综合分析表明,在MAR index≥0.7的41个耐药菌中,75.61%(31/41)的A.hydrophila携带3个及以上毒力基因。研究结果证实A.hydrophila在不同来源样本中普遍存在,且表现出多样的毒力因子组合以及普遍的多重耐药性。
In order to understand the distribution of virulence factors of A.hydrophila and its drug resistance phenotype,102 animal samples and 15 water environment samples were collected in this study,and A.hydrophila was isolated,cultured and identified.Subsequently,five virulence genes of ear,alt,hly,lip and act were determined by PCR,and the sensitivity of A.hydrophila to 10 drugs such as cefotaxime,ceftriaxone and ofloxacin was determined by Kirby-Bauer disk diffusion method.The results showed that 116 A.hydrophila strains from fish,43 A.hydrophila strains from frog,11 A.hydrophila strains from snake and 16 A.hydrophila strains from water were isolated from 117 samples.The positive detection rate of virulence gene act was the highest(73.65%),followed by ear(65.59%),and the most common virulence genotype was ear+hly+act,and 75.81%of A.hydrophila carried at least three or more virulence genes.All 186 strains of A.hydrophila were resistant to two or more antimicrobials,the multiple resistance rate was 100%,the MAR index was 0.2-0.9,and the"AMX+AMP+VA+GEN"resistance type was the most common.Comprehensive analysis of drug resistance phenotypes and virulence genotypes showed that 75.61%(31/41)of A.hydrophila carried 3 or more virulence genes among 41 strains with MAR index≥0.7.The research results showed that A.hydrophila was common in samples from different sources,and showed various combinations of virulence factors and widespread multi-drug resistance.
作者
田堯
姜艳霞
魏佳欣
王红兵
黎满香
刘俊琦
TIAN Yao;JIANG Yan-xia;WEI Jia-xin;WANG Hong-bing;LI Man-xiang;LIU Jun-qi(College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Institute of Animal Husbandry and Veterinary Science,Changsha,Hunan 410131,China)
出处
《动物医学进展》
北大核心
2025年第10期26-34,共9页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(32302933)。
关键词
嗜水气单胞菌
毒力基因
耐药性
Aeromonas hydrophila
Virulence gene
Drug resistance
