摘要
目的探讨血浆甲基化ZNF582联合ELMO1对胃癌(GC)诊断的可行性。方法收集本院47例GC患者和63例无相关胃肠疾病患者的血浆作为GC组和对照组,用实时荧光定量PCR(qPCR)检测甲基化标志物ZNF582与ELMO1。结果GC组ZNF582的甲基化水平极显著高于对照组(P<0.001),ELMO1甲基化水平显著高于对照组(P<0.05)。ELMO1的敏感性与GC浸润深度和分期显著相关,T3~T4期高于T1~T2期(12.5%vs 51.6%),Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期(22.7%vs 52.0%)。与Ⅰ~Ⅱ期的GC相比,Ⅲ~Ⅳ期GC中ZNF582的敏感性更高(27.3%vs 56.0%)。当甲基化ZNF582和ELMO1相结合,敏感性较单独检测均增加,且甲基化ZNF582和ELMO1在T3~T4期和Ⅲ~Ⅳ期敏感性较T1~T2期和Ⅰ~Ⅱ期高(31.2%vs 71.0%,36.4%vs 68.0%),差异均有统计学意义(P<0.05)。ROC曲线分析显示甲基化ZNF582和ELMO1用于区分GC组和对照组的ROC曲线下面积(AUC)值分别为0.7270(95%CI:0.6277~0.8263)和0.7789(95%CI:0.6398~0.9180)。当两者联合,AUC提高至0.8990(95%CI:0.8411~0.9570)。结论血浆ZNF582和ELMO1的甲基化水平高于对照组,在检测GC有较高的敏感性,且与浸润深度和临床分期有关,将两个甲基化组合可提高诊断GC的准确性。故血浆ZNF582和ELMO1用于GC的诊断和预测病理学分期中有潜在临床意义。
Objective To investigate the feasibility of using plasma methylation of ZNF582 in conjunction with ELMO1 to diagnose gastric cancer(GC).Methods Plasma samples from 47 GC patients and 63 individuals without relevant gastrointestinal diseases were collected as the experimental and control groups,respectively,at The Affiliated Jiangning Hospital of Nanjing Medical University.Methylation markers ZNF582 and ELMO1 were quantified using Quantitative Real-time Polymerase Chain Reaction(qPCR).Results The methylation level of ZNF582 in the GC group was significantly higher than that in the control group(P<0.0001),and the ELMO1 methylation level was significantly elevated in the GC group compared to the control group(P<0.05).The sensitivity of ELMO1 correlated significantly with the depth of invasion and staging of GC,with higher sensitivity in stages T3-T4 compared to T1-T2(12.5%vs 51.6%),and in stages Ⅲ-Ⅳ compared toⅠ-Ⅱ(22.7%vs 52%).Compared to stagesⅠ-Ⅱ GC,stages Ⅲ-Ⅳ GC showed higher sensitivity for ZNF582(27.3%vs 56.0%).When methylation of ZNF582 and ELMO1 were combined,sensitivity increased,compared to individual detection.Additionally,the methylation of ZNF582 and ELMO1 demonstrates higher sensitivity for T3-T4 and Ⅲ-Ⅳ stages than for T1-T2 and Ⅰ-Ⅱ stages(31.2%vs 71.0%,36.4%vs 68.0%,respectively),with statistically significant differences (P<0.05).The ROC curve analysis revealed that area under curve(AUC)values for methylation of ZNF582 and ELMO1 in distinguishing the GC group from the control group were 0.7270(95%CI:0.6277-0.8263)and 0.7789(95%CI:0.6398-0.9180),respectively.When combined,the AUC increased to 0.8990(95%CI:0.8411-0.9570).Conclusion The plasma methylation levels of ZNF582 and ELMO1 are higher than those in the control group,exhibiting heightened sensitivity in detecting GC,and showing an association with clinical staging.Combining these two methylation markers enhances the precision of GC diagnosis.Therefore,the utilization of plasma ZNF582 and ELMO1 in diagnosing GC and predicting pathological staging holds potential clinical significance.
作者
彭程
陆程灿
贺奇彬
甘露
苗莹莹
朱珠
郑永娉
王慧
PENG Cheng;LU Chengcan;HE Qibin;GAN Lu;MIAO Yingying;ZHU Zhu;ZHENG Yongping;Wang Hui(Department of Gastroenterology,Jiangning Hospital Affiliated to Nanjing Medical University,Nanjing 211100,China;Department of Pharmacy,Jiangning Hospital Affiliated to Nanjing Medical University,Nanjing 211100,China)
出处
《西部医学》
2025年第9期1316-1320,共5页
Medical Journal of West China
基金
江苏省药学会-“药”研新声药学科研项目(202495074)
江苏卫生健康职业学院-面上项目(JKC2022059)
南京医科大学附属江宁医院青年创新科研基金项目(JNYYZXKY202417)。
