摘要
目的探讨胰腺导管腺癌(PDAC)中粘蛋白1(MUC1)的表达水平与Notch信号通路中Notch1受体的表达水平的相关性,为研究PDAC发生发展的分子机制提供新方向。方法收集2017年10月—2024年2月在本院行手术治疗的35例PDAC患者的病理石蜡组织,使用免疫组织化学技术检测PDAC组织及其邻近癌旁组织中MUC1与Notch1的表达量,并通过卡方检验来评估两者表达差异的显著性。用Spearman法分析PDAC组织中MUC1和Notch1表达水平的相关性。通过t检验和Fisher精确检验探究MUC1与Notch1的表达水平与患者临床病理特征之间的关联性。结果免疫组织化学分析表明,相较于癌旁组织,MUC1在癌组织中的表达显著升高(χ^(2)=11.497,P=0.001);同样,Notch1在癌组织中的表达也明显高于癌旁组织(χ^(2)=23.026,P<0.001)。PDAC组织中MUC1和Notch1的表达水平呈正相关(r=0.652,P<0.001)。MUC1的表达与PDAC分化程度有关(P=0.018)。Notch1的表达与PDAC患者年龄(t=3.17,P=0.005)、性别(P=0.012)、分化程度(P=0.027)有关。结论在PDAC中MUC1和Notch1的表达均上调,且两者表达水平呈正相关,提示MUC1可能通过调控Notch信号通路来影响PDAC的发生发展。MUC1和Notch1均与PDAC分化程度有关,随着肿瘤分化程度升高,MUC1和Notch1表达逐渐降低,提示两者的表达可能与PDAC的恶性程度和疾病预后有关。
Objective To explore the relationship between mucin 1(MUC1)expression and Notch1 receptor expression in pancreatic ductal adenocarcinoma(PDAC)and provide a fresh perspective on investigating the molecular mechanisms underlying PDAC onset and progression.Methods From October 2017 to February 2024,paraffin-embedded specimens from 35 surgically treated PDAC patients at Shanxi Provincial People's Hospital were gathered.Immunohistochemical analysis was conducted to assess MUC1 and Notch1 expression levels in both PDAC tissues and adjacent tissues.The statistical significance of expression differences between these tissues was evaluated using the chi-square test.Spearman's rank correlation coefficient was employed to analyze the relationship between MUC1 and Notch1 expression in PDAC tissues.Additionally,the t-test and Fisher's exact test were utilized to investigate the association between MUC1 and Notch1 expression levels and the clinicopathological features of the patients.Results Immunohistochemical analysis demonstrated a significant increase in MUC1 expression in cancer tissues compared to adjacent non-cancerous tissues(χ^(2)=11.497,P=0.001).Notch1 expression was notably elevated in cancer tissues versus adjacent tissues(χ^(2)=23.026,P<0.001).A positive correlation was found between MUC1 and Notch1 expression levels in PDAC tissues(r=0.652,P<0.001).MUC1 expression correlated with the degree of PDAC differentiation(P=0.018).Furthermore,Notch1 expression was associated with patient age(t=3.17,P=0.005),gender(P=0.012),and PDAC differentiation level(P=0.027).Conclusion The expressions of MUC1 and Notch1 were enhanced in PDAC,with MUC1 levels positively correlated with those of Notch1,hinting that MUC1 may play a role in PDAC onset and progression by modulating the Notch signaling pathway.Both MUC1 and Notch1 are related to the degree of PDAC differentiation.With the increase of tumor differentiation,the expressions of MUC1 and Notch1 gradually decrease,suggesting that the expression of both may be related to the malignant degree and disease prognosis of PDAC.
作者
白杨
庞捷
范大光
BAI Yang;PANG Jie;FAN Daguang(Department of General Surgery,Shanxi Provincial People's Hospital,Shanxi Medical University,Taiyuan 030012,China)
出处
《西部医学》
2025年第9期1269-1273,共5页
Medical Journal of West China
基金
山西省基础研究计划项目(202103021224381)。
