摘要
以金花茶为试材,采用生物信息学方法,研究了YABBY基因家族的全基因组鉴定及功能分析,以期为金花茶资源开发及花器官发育分子机制研究提供参考依据。结果表明:在金花茶基因组中共鉴定出9个YABBY基因(CnYABBY1~CnYABBY9),可分为4个亚家族,分布于7条染色体上,成员间序列保守,且均预测定位于细胞核。启动子顺式作用元件分析显示,光响应元件最为丰富,暗示其可能参与调控光合作用;此外还富含脱落酸、厌氧及茉莉酸甲酯等响应元件。不同花期表达量表明,除CnYABBY3和CnYABBY5外,其他成员均在花发育不同时期的花器官中表达。其中,CnYABBY1在雌蕊发育中持续高表达,并在萼片发育早期(Ⅰ、Ⅱ期)显著高表达,提示其可能是调控雌蕊发育及萼片形态建成的关键基因。综上所述,金花茶YABBY基因家族在光合作用及花器官(尤其雌蕊与萼片)发育中发挥重要作用,CnYABBY1是其中的关键调控因子。
Taking Camellia nitidissima as experimental material,this study employed bioinformatics methods to conduct a genome-wide identification and functional analysis of the YABBY gene family,in order to provide reference for the development of Camellia nitidissima resources and the research on the molecular mechanism of floral organ development.The results showed that nine YABBY genes(CnYABBY1-CnYABBY9)were identified in the Camellia nitidissima genome,classified into four subfamilies and distributed across seven chromosomes.The members exhibited conserved sequences and were all predicted to be localized in the nucleus.Promoter cis-acting element analysis revealed that light-responsive elements were the most abundant,suggesting potential involvement in photosynthesis regulation;moreover,elements responsive to abscisic acid,anaerobic conditions,and methyl jasmonate were also enriched.Expression analysis across different flowering stages indicated that,except forCnYABBY3 and CnYABBY5,all other members were expressed in floral organs at various developmental stages.Notably,CnYABBY1was persistently highly expressed during pistil development and significantly highly expressed in the early stages(stagesⅠandⅡ)of sepal development,indicating its potential role as a key regulatory gene in pistil development and sepal morphogenesis.In conclusion,the YABBY gene family in Camellia nitidissima plays crucial roles in photosynthesis and floral organ(particularly pistil and sepal)development,withCnYABBY1being a key regulatory factor.
作者
张梓扬
陈德星
李文卉
林宇清
孙维红
邹双全
ZHANG Ziyang;CHEN Dexing;LI Wenhui;LIN Yuqing;SUN Weihong;ZOU Shuangquan(College of Forestry,Fujian Agriculture and Forestry University/Engineering Research Center of Natural Biological Resources Conservation&Utilization of Fujian Province,Fuzhou,Fujian 350002;School of Big Data,Fuzhou University of Foreign Languages and Trade,Fuzhou,Fujian 350202)
出处
《北方园艺》
北大核心
2025年第17期1-9,共9页
Northern Horticulture
基金
福建省自然科学基金资助项目(2023J05216)。
