摘要
MAPK(促分裂原活化蛋白激酶)信号转导通路是一种广泛存在于真核生物中的重要信号转导机制,通过磷酸化逐级激活MAPKKK、MAPKK和MAPK三个关键蛋白激酶家族来调控细胞对外界刺激做出相应的生理响应。为了研究OsMAPK5(登录号LOC_Os03g17700)基因在水稻中的功能,我们通过CRISPR/Cas9基因编辑和转基因技术创制了OsMAPK5基因敲除突变体和过表达材料。成熟期表型鉴定结果表明,OsMAPK5基因能够矮化水稻株高,其中OsMAPK5基因功能缺失通过抑制穗长与倒一节间长实现,而过表达OsMAPK5基因则通过抑制穗长和倒一节间长、倒二节间长实现。进一步研究发现,OsMAPK5还影响水稻穗发育和结实,过表达OsMAPK5基因使得水稻一次枝梗数、二次枝梗数、穗粒数、结实率均显著减少,而OsMAPK5功能缺失对水稻穗发育的影响不显著,但显著降低结实率。综上,OsMAPK5基因具有矮化水稻株高的功能,且影响穗的发育和结实,这一发现有助于深入完善水稻株高的分子调控机制,并为育种工作提供基因资源。
The MAPK(mitogen-activated protein kinase)signaling pathway is an important signaling mechanism widely present in eukaryotes,which regulates the physiological response of cells to external stimuli through gradually activating three key protein kinase families,MAPKKK,MAPKK and MAPK by phosphorylation.In order to study the function of OsMAPK5(accession number:LOC_Os03g17700)gene in rice,we created the OsMAPK5 gene knockout mutants and overexpression materials by CRISPR/Cas9 gene editing and transgenic technology.The results of phenotypic identification at maturity showed that the OsMAPK5 gene could dwarf the plant height of rice,and the loss of OsMAPK5 gene function was achieved by inhibiting the panicle length and the inverted internode length,while the overexpression of OsMAPK5 gene was achieved by inhibiting the length of panicle and the first and second inverted internodes.Further studies showed that OsMAPK5 also affected panicle development and seed setting in rice,and overexpression of OsMAPK5 gene significantly reduced the number of primary branches,secondary branches,grains per panicle and seed setting rate.However,the loss of OsMAPK5 function had no significant effect on panicle development,but significantly reduced the seed setting rate.In conclusion,the OsMAPK5 gene had the function of dwarfing rice plant height,and affected panicle development and seed setting,which would be helpful to further improve the molecular regulation mechanism of rice plant height and provide gene resources for breeding.
作者
刘泽赟
周杰强
陈文娟
董雅婷
王姝洁
张俊博
余强
唐文帮
肖云华
Liu Zeyun;Zhou Jieqiang;Chen Wenjuan;Dong Yating;Wang Shujie;Zhang Junbo;Yu Qiang;Tang Wenbang;Xiao Yunhua(College of Agronomy,Hunan Agricultural University,Changsha 410128,China;Key Laboratory of Rice and Rape Disease Resistance Breeding of Hunan Province,Changsha 410128,China;Stale Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410128,China;National Technology Innovation Center for Salt-Tolerant Rice,Changsha 410128,China)
出处
《山东农业科学》
北大核心
2025年第8期8-14,共7页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(32172078)
湖南省科技创新计划项目(2023NK1010)。
