摘要
[目的]为查明山东省某规模化奶牛场腹泻犊牛病因及病原体耐药性,研究采集病料进行病原学鉴定和耐药性分析。[方法]无菌采集病死犊牛肠系膜、肺脏、脾脏等组织病料装入采样袋,共18份,通过细菌分离培养及生化试验,细菌16S rRNA进行PCR扩增和毒素分型鉴定,运用K-B琼脂扩散纸法对分离菌株耐药性试验,PCR电泳检测14种耐药基因。[结果]结果显示,5株C型产气荚膜梭菌被检出,检出率为27.8%。分离菌对多种抗生素表现耐药性,β-内酰胺类、氨基糖苷类、磺胺类和氯霉素类耐药基因均有检出。[结论]研究结果表明,C型产气荚膜梭菌是引起奶牛场腹泻犊牛发病的致病菌,且对部分抗生素表现耐药性。该研究结果为奶牛场产气荚膜梭菌防控提供参考。
[Objective]In order to find out the etiology and pathogen resistance of diarrhea calves in a largescale dairy farm in Shandong Province,the pathogenic identification and drug resistance analysis were carried out.[Method]A total of 18 samples of mesenteric,lung,spleen and other tissues of dead calves were collected aseptically and put into sampling bags.Through bacterial isolation and culture and biochemical test,bacterial 16 S rRNA was amplified by PCR and toxin typing was identified.The K-B agar diffusion paper method was used to test the drug resistance of the isolated strains,and 14 drug resistance genes were detected by PCR electrophoresis.[Result]The results showed that 5 strains of C.perfringens type C were detected,and the detection rate was 27.8%.The isolated bacteria showed resistance to a variety of antibiotics,andβ-lactams,aminoglycosides,sulfonamides and chloramphenicol resistance genes were detected.[Conclusion]The results showed that C.perfringens type C was the pathogenic bacteria causing diarrhea in calves on dairy farms,and it was resistant to some antibiotics.The results of this study provide a reference for the prevention and control of Clostridium perfringens in dairy farms.
作者
刘芝美
LIU Zhimei(Da Zhuang Animal Husbandry and Veterinary Station,Yinan County,Linyi Shandong 276305)
出处
《中国乳业》
2025年第8期54-60,共7页
China Dairy
关键词
腹泻犊牛
产气荚膜梭菌
毒素基因分型
耐药性
diarrhea calves
clostridium perfringens
toxin genotyping
drug resistance
