摘要
【目的】二酰基甘油酰基转移酶(Diacylglycerol acyltransferase,DGAT)是三酰甘油(Triacylglycerol,TAG)合成途径的关键酶之一。通过探究普通油茶DGAT基因家族在不同组织及不同逆境下的功能作用,为进一步阐明DGAT基因功能奠定基础。【方法】利用生物信息学方法对普通油茶DGAT基因家族进行鉴定,对其理化性质、系统进化、基因结构、顺式作用调控元件、复制事件、共线性及表达模式进行分析。【结果】在普通油茶基因组中鉴定到23个DGAT基因,其编码蛋白质长度在215~514个氨基酸之间,分子量在24.09~58.39 kDa之间,等电点在6.37~9.64之间,亚细胞定位主要在质膜和叶绿体中。拟南芥、大豆、花生、茶树和油茶的66个DGAT蛋白聚类为4个亚族。基因结构分析表明油茶DGAT基因家族有较高的保守性,顺式作用元件分析表明油茶DGAT基因启动子含有大量植物生长发育、植物激素响应以及逆境胁迫元件。油茶DGAT基因家族之间的共线性均存在于同源染色体之间,且纯化选择在进化中起重要作用。不同组织及处理条件下的转录表达分析表明,B1.7G155100、C2.7G153700、C1.13G060000基因在胚胎的中后期表达量最高,可能参与种仁后期脂肪酸合成;而A1.7G177400、C1.7G167500、C2.7G152700、A1.4G216200、C2.7G153800则在茎、叶和花芽中优势表达,推测参与上述组织的蜡质形成过程。A1.7G177400、C2.7G152700、B1.7G155300、C1.7G166400、C1.7G1675005个基因及C1.1G198100、C1.2G252700、A1.4G0739003个基因分别参与炭疽病菌和低温胁迫的响应过程。【结论】23个DGAT基因家族成员在进化上相对保守,在不同组织中表达模式不同,推测在生长发育、抗炭疽病菌和低温胁迫等方面发挥着重要作用。
[Objective]Diacylglycerol acyltransferas(DGAT)is a key enzyme in the synthesis pathway of triglyceride(TAG).The present study aimed to investigate the roles of the DGAT gene family in different tissues and various stress conditions in Camellia oleifera,providing a theoretical foundation for further elucidating the functions of DGAT genes.[Method]The bioinformatics methods was used to identify and analyze the DGAT gene family in C.oleifera,and physicochemical properties,phylogenetic evolution,gene structure,cis-acting regulatory elements,duplication events,collinearity and expression patterns were analyzed.[Result]A total of 23 DGAT genes were identified from the C.oleifera genome.The lengths of protein sequences of DGAT ranged from 215 to 514 amino acids.The molecular weights ranged from 24.09 to 58.39 kilodaltons(kDa).The isoelectric points ranged from 6.37 to 9.64.And the predicted subcellular localizations of DGAT genes were mainly located in chloroplast and plasma membrane.Through phylogenetic analysis of family genes,the DGAT gene family members were clus-tered into IV clades.Gene structure analysis indicated that the DGAT gene family had a high degree of conservation in C.oleijfera.Cis-acting element analysis showed that the promoters of the DGAT genes contained a large number of elements related to plant growth and development,plant hormone response,as well as adversity stress in C.oleifera.The collinearity among the DGAT genes existed only between homologous chromosomes,and purifying selection played an important role in evolution.Transcriptional expression analysis under different organizations and treatments revealed that the genes B1.7G155100,C2.7G153700 and C1.13G060000 exhibited the highest expression levels during the middle and late stages of embryo development,potentially involved in the synthesis of fatty acids in the later stages of seed development.In contrast,the genes A1.7G177400,CI.7G167500,C2.7G152700,AI.4G216200 and C2.7GI153800 showed dominant expression in stems,leaves and flower buds,suggesting that these genes were involved in the wax formation process in these tissues.Additionally,five genes,namely AI.7G177400,C2.7G152700,B1.7G155300,CI.7G166400 and C1.7G167500,three genes,namely C1.1G198100,CI.2G252700 and Al.4G073900,were involved in the response processes of Colletotrichum gloeosporioides and low-temperature stress,respectively.[Conclusion]The 23 members of the DGAT gene family are relatively conserved in terms of evolution and exhibit distinct expression patterns across different tssues,suggesting that they play significant roles in growth and development,resistanceto anthracnoseand response tolow-tempera ture stress.
作者
汤欣欣
黄欣仪
胡孝义
邓钰彬
胡孝明
朱华国
TANG Xin-xin;HUANG Xin-yi;HU Xiao-yi;DENG Yu-bin;HU Xiao-ming;ZHU Hua-guo(College of Biology and Agricultural Resources,Huanggang Normal University,Huanggang,Hubei 438000,China;Huanggang Normal University/Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Uilization,Huanggang,Hubei 438000,China;School of Forestry&Landscape Architecture,Anhui Agricultural University,Hefei 230036,China)
出处
《西南农业学报》
北大核心
2025年第6期1209-1218,共10页
Southwest China Journal of Agricultural Sciences
基金
湖北省自然科学基金联合基金项目(2025AFD333)
湖北省高等学校优秀中青年科技创新团队(T2023025)
湖北省中央引导地方科技发展专项(2024CSA071)。
关键词
油茶
二酰基甘油酰基转移酶
全基因组分析
表达分析
Camellia oleifera
Diacylglycerol acyltransferas
Whole-genome analysis
Gene expression
