摘要
目的:探讨葛根芩连汤(GQD)对非酒精性脂肪性肝炎(NASH)大鼠抗炎和抗氧化应激及相关通路的影响,并阐明其作用机制。方法:采用蛋氨酸胆碱缺乏饲料建立NASH大鼠模型,分别设置空白组、模型组、阳性组和不同剂量GQD给药组,通过分析大鼠血清炎症指标、肝组织抗氧化能力指标、肝组织病理切片,并结合Western blot和RT-qPCR技术,阐明GQD对NASH大鼠抗炎和抗氧化应激的作用机制。结果:各剂量GQD均可显著降低NASH模型大鼠血清IL-6、IL-8、IL-10、IFN-α、IFN-β含量,且GQD中、高剂量组与模型组、阳性组相比差异均有统计学意义;各剂量GQD均可显著降低NASH大鼠肝组织ALT、AST、MDA含量,同时显著提升其SOD、GPX、T-AOC含量;Western blot和RT-qPCR结果显示,GQD可显著降低NASH模型大鼠肝组织PI3K、AKT、p-FOXO1蛋白和mRNA表达,显著提高FOXO1、SIRT1、Catalase蛋白和mRNA表达,且高剂量给药时与阳性组相比差异有统计学意义。结论:GQD主要通过调控PI3K/AKT/FOXO1信号通路发挥抗炎和抗氧化应激作用治疗NASH。
Objective:To investigate influence of Gegen Qinlian Decoction(GQD)on anti-inflammatory,anti-oxidative stress activities and related pathways in rats with non-alcoholic steatohepatitis(NASH),and to clarify its mechanism.Methods:NASH rat model was established with methionine choline deficiency feed,and blank group,model group,positive group and different doses of GQD administration groups were set.By analyzing rat serum inflammation indicators,liver tissue antioxidant capacity indicators,liver tissue pathological sections,and combined with Western blot and RT-qPCR technology,anti-inflammatory and anti-oxidative stress mechanism of GQD on NASH rats were finally clarified.Results:Each dose of GQD could significantly reduce serum levels of IL-6,IL-8,IL-10,IFN-αand IFN-βin NASH model rats.GQD medium and high doses groups were statistically significant compared with model group and positive group;each dose of GQD could significantly reduce contents of ALT,AST and MDA in liver tissue of NASH rats,and significantly increase SOD,GPX,T-AOC contents at the same time;Western blot and RT-qPCR results showed that GQD could significantly reduce expressions of PI3K,AKT,p-FOXO1 protein and mRNA in liver tissue of NASH model,significantly increase expressions of FOXO1,SIRT1,Catalase protein and mRNA,and compared with positive group at high doses,it was also statistically significant.Conclusion:GQD can treat NASH by regulating PI3K/AKT/FOXO1 signaling pathway and exerting antiinflammatory and anti-oxidative stress effects.
作者
张超云
费娜
郝鹏飞
母梦雨
张仲鸣
ZHANG Chaoyun;FEI Na;HAO Pengfei;MU Mengyu;ZHANG Zhongming(ZHANG Zhongjing School of Chinese Medicine,Nanyang Institute of Technology,Nanyang 473004,China;Henan Technician College of Medicine and Health,Kaifeng 475000,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期1895-1900,共6页
Chinese Journal of Immunology
基金
河南省科技发展计划项目(212102311102)。
关键词
葛根芩连汤
非酒精性脂肪肝炎
抗炎
抗氧化应激
Gegen Qinlian Decoction
Non-alcoholic steatohepatitis
Anti-inflammatory
Anti-oxidative stress
