摘要
目的:研究浙贝母提取物协同急性炎症刺激对非小细胞肺癌的生长抑制作用。方法:制备浙贝母提取物并采用UPLC-QE/MS对其进行化学成分表征。复制Lewis肺癌异位荷瘤小鼠模型,注射酵母菌诱发急性炎症,比较浙贝母提取物协同急性炎症对Lewis肺癌小鼠生长情况、肿瘤体积和抑瘤率的影响,并采用流式CBA法测定小鼠肿瘤组织中炎症因子(IL-6、IL-1β、IL-1α、IL-10、IL-27、IL-17A、IL-12p70、IL-23、TNF-α、IFN-γ、IFN-β、GM-CSF、MCP-1)含量的变化。结果:酵母菌刺激引起急性炎症后对Lewis肺癌小鼠的抑制与单用顺铂的效果相近,抑瘤率为35%;浙贝母提取物联合酵母菌急性炎症刺激的抑瘤率为62%,是单用顺铂的1.8倍。酵母菌急性炎症刺激后细胞因子IL-23、MCP-1表达量的降低与肿瘤抑制有关;而IL-6、IL-1β、IL-1α、IL-10、IL-27、IL-17A、IL-12p70、TNF-α、IFN-γ、IFN-β、GM-CSF细胞因子表达量的升高与抑制肿瘤相关。结论:浙贝母提取物协同急性炎症刺激可起到积极的抗非小细胞肺癌作用,这将为非小细胞肺癌防治提供新的研究思路和方法。
Objective:To study the growth inhibition of Thunberg Fritillary extract on non-small cell lung cancer.Methods:The Thunberg Fritillary extract was prepared and characterized by UPLC-QE/MS.Replicated Lewis lung carcinoma ectopic tumor-bear-ing mouse model,yeast injection induced acute inflammation,compared the effect of Thunberg Fritillary extract combination with acute inflammation on the growth,tumor volume and tumor suppression rate of Lewis lung carcinoma mice,and determine the content of inflammatory factors by the flow CBA method(IL-6,IL-1β,IL-1α,IL-10,IL-27,IL-17A,IL-12p70,IL-23,TNF-α,IFN-γ,IFN-β,GM-CSF,MCP-1).Results:The inhibition of Lewis lung carcinoma mice was similar to that of cisplatin alone,and the tumor suppression rate was 35%;the tumor suppression rate of Thunberg Fritillary extract combined with acute inflammatory stimulation of yeast was 62%,1.8 times that of cisplatin alone.The decrease in the expressions of cytokines IL-23,MCP-1 after acute inflammatory stimulation in yeast was associated with tumor suppression;while the increased expressions of IL-6,IL-1β,IL-1α,IL-10,IL-27,IL-17A,IL-12p70,TNF-α,IFN-γ,IFN-βand GM-CSF cytokines were associated with tumor suppression.Conclusion:The Thun-berg Fritillary extract combination with acute inflammation can play a positive role against non-small cell lung cancer,which will pro-vide new research ideas and methods for the prevention and treatment of non-small cell lung cancer.
作者
王含雪
邢淑雁
杨佳
刘孝云
叶冬雪
张国英
容蓉
杨勇
WANG Hanxue;XING Shuyan;YANG Jia;LIU Xiaoyun;YE Dongxue;ZHANG Guoying;RONG Rong;YANG Yong(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Key Laboratory of Classical Theory of Traditional Chinese Medicine,Ministry of Education,Jinan 250355,China;Shandong Engineering Research Center for Antiviral of Traditional Chinese Medicine,Jinan 250355,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期1800-1805,共6页
Chinese Journal of Immunology
基金
国家自然科学基金青年科学基金(21807066)。
关键词
LEWIS肺癌
急性炎症
浙贝母
非小细胞肺癌
Lewis lung carcinoma
Acute inflammation
Thunberg Fritillary
Non-small cell lung cancer
