摘要
目的:观察白芷-川芎通过脑肠轴途径对血管性痴呆(VD)模型大鼠行为学的影响,探讨白芷-川芎对VD大鼠的神经保护作用。方法:60只SPF级SD大鼠,选取12只作为对照组,其他构建VD模型,共36只大鼠建模成功,分为模型组、阳性药物组、白芷-川芎组,每组12只。观察各组大鼠病理组织学、空间学习记忆能力、血管新生、脑肠肽指标、免疫及炎症指标、SIRT1/FOXO3A通路mRNA、蛋白表达。结果:与对照组相比,模型组游泳总路程、穿越平台时间增加,穿越平台数减少,ET-1、NO、iNOS、CD8^(+)T、TNF-α、IL-1β、IL-8表达、FOXO3A mRNA、蛋白表达升高,VEGF、MTL、GAS、VIP、CD4^(+)T/CD8^(+)T、CD4^(+)T表达、SIRT1 mRNA、蛋白表达降低(P<0.05);与模型组相比,阳性药物组、白芷-川芎组游泳总路程、穿越平台时间减少,穿越平台数增加,ET-1、NO、iNOS、CD8^(+)T、TNF-α、IL-1β、IL-8表达、FOXO3A mRNA、蛋白表达降低,VEGF、MTL、GAS、VIP、CD4^(+)T/CD8^(+)T、CD4^(+)T表达、SIRT1 mRNA、蛋白表达升高(P<0.05);与阳性药物组相比,白芷-川芎组游泳总路程、穿越平台时间减少,穿越平台数增加,ET-1、NO、iNOS、CD8^(+)T、TNF-α、IL-1β、IL-8表达、FOXO3A mRNA、蛋白表达降低,VEGF、MTL、GAS、VIP、CD4^(+)T/CD8^(+)T、CD4^(+)T表达、SIRT1 mRNA、蛋白表达升高(P<0.05)。结论:白芷-川芎可调控脑肠轴途径,促进脑肠肽指标表达,改善VD大鼠学习记忆能力,保护神经元。
Objective:To observe effect of Angelica dahurica-Chuanxiong on behavior of vascular dementia(VD)model rats through cerebral and intestinal axis pathway,and to explore neuroprotective effect of Angelica dahurica-Chuanxiong on VD rats.Methods:A total of 60 SD rats with SPF grade were selected,and 12 rats were selected as control group,VD models were constructed in another rats,and 36 rats were successfully modeled,which were divided into model group,positive drug group and Angelica dah-urica-Chuanxiong group,with 12 rats in each group.Histopathology,spatial learning and memory ability,angiogenesis,brain-intesti-nal peptide index,immune and inflammatory indicators,SIRT1/FOXO3A pathway mRNA and protein expressions were observed in each group.Results:Compared with control group,model group showed an increase in total swimming distance and crossing platform time,a decrease in number of crossing platforms,an increase in ET-1,NO,iNOS,CD8^(+)T,TNF-α,IL-1β,IL-8 expressions and FOXO3A mRNA and protein expressions,and a decrease in VEGF,MTL,GAS,VIP,CD4^(+)T/CD8^(+)T,CD4^(+)T expression,SIRT1 mRNA and protein expressions were decreased(P<0.05);compared with model group,positive drug group and Angelica dahurica-Chuanxiong group showed a decrease in total swimming distance and crossing platform time,an increase in number of crossing plat-forms,a decrease in ET-1,NO,iNOS,CD8^(+)T,TNF-α,IL-1β,IL-8 expressions and FOXO3A mRNA and protein expressions,and a decrease in VEGF,MTL,GAS,VIP,CD4^(+)T/CD8^(+)T,CD4^(+)T expression,SIRT1 mRNA and protein expressions were elevated(P<0.05);compared with positive drug group,total swimming distance and crossing platform time were decreased,crossing platform number was increased,ET-1,NO,iNOS,CD8^(+)T,TNF-α,IL-1β,IL-8 expressions,FOXO3A mRNA and protein expressions were decreased,VEGF,MTL,GAS,VIP,CD4^(+)T/CD8^(+)T,CD4^(+)T expression,SIRT1 mRNA and protein expressions were increased in Angelica dahurica-Chuanxiong group(P<0.05).Conclusion:Angelica dahurica-Chuanxiong can regulate brain-gut axis pathway,pro-mote expression of brain-gut peptide index,improve learning and memory ability of VD rats,and protect neurons.
作者
李健
刘明
刘杨
邓颖
杨欣
LI Jian;LIU Ming;LIU Yang;DENG Ying;YANG Xin(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期1853-1858,共6页
Chinese Journal of Immunology
基金
2023年贵州省高等学校重点实验室科研项目(黔教技[2023]018号)
2022年贵州省教育厅滚动支持省属高校科研平台团队项目(黔教技[2022]022号)
贵州中医药大学校级研究中心项目(贵中医ZX合字[2024]024号)。
关键词
血管性痴呆
白芷-川芎
脑肠轴
神经保护
Vascular dementia
Angelica dahurica-Chuanxiong
Cerebral and intestinal axis pathway
Neuroprotection
