摘要
为探究玉米(Zea mays L.)幼苗根系在渍水胁迫下关键代谢物以及代谢途径的变化,阐明与渍水胁迫相关的代谢通路,对玉米幼苗进行0、1、4和7 d共4个渍水胁迫时间梯度处理,采用转录组学测序(RNA sequencing, RNA-seq)和超高效液相色谱质谱联用技术(ultra performance liquid chromatography tandem mass spectrometry, UPLC-MS/MS),筛选与渍水胁迫相关的关键代谢物,并通过KEGG富集分析探究相关代谢通路。结果显示,代谢组共标注和定量了1361种差异代谢物,渍水4 d时根系代谢物变化最为显著,共有414种代谢物发生变化,其中372种代谢物含量上升,42种代谢物含量下降;代谢物差异倍数排名前20的代谢物均呈现上调趋势,包括阿魏酸-4-O-葡萄糖苷、2-苯乙醇、7-甲基柚皮素和S-烯丙基-L-半胱氨酸等;差异显著代谢物的KEGG富集分析结果显示,黄酮类化合物生物合成、类胡萝卜素生物合成、苯丙素生物合成、植物激素信号转导、ABC转运蛋白、脂肪酸降解、淀粉和蔗糖代谢、甘氨酸、丝氨酸和苏氨酸代谢、色氨酸代谢以及糖酵解/糖异生等代谢途径中,差异基因与差异代谢物较为显著富集。类黄酮生物合成代谢通路与渍水胁迫密切相关,柚皮素与木犀草素等黄酮类物质,查尔酮异构酶、黄酮合成酶Ⅱ与黄酮3',5'-羟化酶/黄酮3'-单氧化酶可能起关键作用。研究结果为探究玉米耐渍胁迫分子机制提供科学支撑与理论依据,为玉米耐渍育种等相关研究提供理论基础。
To investigate key metabolites and changes in metabolic pathways in maize(Zea mays L.)seedlings under waterlog-ging stress,plants were subjected to four stress durations(0,1,4,and 7 days).Transcriptome sequencing(RNA-seq)and ul-tra-performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS)were employed to identify differentially accumulated metabolites,followed by KEGG enrichment analysis to explore associated metabolic pathways.A total of 1361 dif-ferential metabolites were annotated and quantified.The most pronounced metabolic changes were observed at 4 days,with 414 metabolites significantly altered—372 upregulated and 42 downregulated.The top 20 most differentially accumulated metabolites were all upregulated,including ferulic acid-4-O-glucoside,2-phenylethanol,7-methyl-ergochalcone,and S-allyl-L-cysteine.KEGG pathway analysis revealed significant enrichment in flavonoid biosynthesis,carotenoid biosynthesis,phenylpropanoid biosynthesis,plant hormone signaling,ABC transporters,fatty acid degradation,starch and sucrose metabolism,glycine,serine and threonine metabolism,tryptophan metabolism,and glycolysis/gluconeogenesis.Among these,flavonoid biosynthesis was found to be closely associated with waterlogging stress,with key flavonoids such as naringenin and luteolin,and enzymes includ-ing chalcone isomerase,flavonoid synthase II,and flavonoid 3',5'-hydroxylase/3'-monooxygenase playing critical roles.These findings provide new insights into the molecular mechanisms underlying maize tolerance to waterlogging and offer a theoretical basis for breeding waterlogging-tolerant maize varieties.
作者
蒋环琪
段奥
郭超
黄晓梦
艾德骏
刘小雪
谭静怡
彭成林
李曼菲
杜何为
JIANG Huan-Qi;DUAN Ao;GUO Chao;HUANG Xiao-Meng;AI De-Jun;LIU Xiao-Xue;TAN Jing-Yi;PENG Cheng-Lin;LI Man-Fei;DU He-Wei(College of Life Sciences,Yangtze University,Jingzhou 434025,Hubei,China;Plant Protection and Soil Fertilizer Institute,Hubei Academy of Agricultural Sciences/National Soil Quality Hongshan Observation and Experimental Station,Wuhan 430064,Hubei,China)
出处
《作物学报》
北大核心
2025年第9期2295-2306,共12页
Acta Agronomica Sinica
基金
国家自然科学基金项目(32072069)
国家自然科学基金青年项目(32301910)
湖北省自然科学基金创新群体项目(2022CFA030)
长江大学大学生创新创业项目(Yz2024251)资助。
关键词
玉米
渍水胁迫
代谢组
差异代谢物
代谢通路
maize
waterlogging stress
metabolomics
differential metabolites
metabolic pathway
