摘要
目的探究细胞增殖相关核抗原Ki67、组蛋白甲基转移酶多梳蛋白增强子同源物2(enhancer of zeste homolog 2,EZH2)在结直肠癌组织中的表达水平及其临床意义。方法回顾性分析南平市第一医院2021年1月—2023年12月收治的72例结直肠癌患者的癌组织及癌旁组织。采用链霉亲和素-生物素复合物(streptavidin-biotin complex,SABC)免疫组织化学法检测Ki67和EZH2蛋白的表达水平,并分析其与结直肠癌临床病理特征的相关性及其诊断效能。结果癌组织的Ki67、EZH2的阳性表达率分别为79.17%和76.39%,高于癌旁组织的19.44%和18.06%,差异有统计学意义(P<0.05)。Logistic回归分析得出,Ki67、EZH2是结直肠癌的危险因素(OR=5.372、5.154)。Ki67、EZH2表达与肿瘤大小、浸润程度及远处转移有相关性,差异有统计学意义(P<0.05),即肿瘤越大、浸润程度越严重、有远处转移患者的Ki67、EZH2阳性表达越高。Ki67高表达结直肠癌患者3年总生存率66.67%低于Ki67低表达患者的93.33%,差异有统计学意义(P<0.05)。EZH2高表达结直肠癌患者3年总生存率70.91%低于EZH2低表达患者的94.12%,差异有统计学意义(P<0.05)。结论Ki67、EZH2是结直肠癌的危险因素,通过对Ki67、EZH2蛋白的联合诊断,可以实现准确的诊断及预后评估。
Objective To explore the expression levels and clinical significance of cell proliferation-associated nuclear antigen Ki67 and histone methyltransferase enhancer of zeste homolog 2(EZH2)in colorectal cancer tissues.Methods The cancerous and adjacent tissues from 72 patients with colorectal cancer admitted to the First Hospital of Nanping City from January 2021 to December 2023 were retrospectively analyzed.The expression levels of Ki67 and EZH2 proteins were detected using the streptavidin-biotin complex(SABC)immunohistochemistry method.The correlation between their expression and the clinicopathological characteristics of colorectal cancer,as well as their diagnostic efficacy,were analyzed.Results The positive expression rates of Ki67 and EZH2 in cancer tissues were 79.17%and 76.39%,respectively,which were significantly higher than those in adjacent tissues(19.44%and 18.06%,respectively),with a statistically significant difference(P<0.05).Logistic regression analysis showed that Ki67 and EZH2 were risk factors for colorectal cancer(OR=5.372,5.154).The expression of Ki67 and EZH2 was significantly correlated with tumor size,invasion depth,with a statistically significant difference(P<0.05),indicating that higher positive expression of Ki67 and EZH2 was observed in patients with larger tumors,more severe invasion,and distant metastasis.The 3-year overall survival rate of colorectal cancer patients with high Ki67 expression was 66.67%,which was significantly lower than that of patients with low Ki67 expression(93.33%),with a statistically significant difference(P<0.05).The 3-year overall survival rate of colorectal cancer patients with high EZH2 expression was 70.91%,which was significantly lower than that of patients with low EZH2 expression(94.12%),with a statistically significant difference(P<0.05).Conclusion Ki67 and EZH2 are risk factors for colorectal cancer.By combining the diagnosis of Ki67 and EZH2 proteins,accurate diagnosis and prognostic evaluation can be achieved.
作者
李新华
陈明光
吴敏敏
LI Xinhua;CHEN Mingguang;WU Minmin(Department of Pathology,Nanping First Hospital,Nanping Fujian 353000)
出处
《中国卫生标准管理》
2025年第11期120-124,共5页
China Health Standard Management
