摘要
目的探究结直肠癌(CRC)组织及淋巴结转移灶中前B细胞白血病同源盒基因3(PBX3)、E-钙黏蛋白(E-cadherin)和人表皮生长因子受体-2(HER-2)的表达及临床意义。方法回顾性分析2021年3月至2024年3月于盐城市第三人民医院就诊的118例CRC患者的临床资料。采用免疫组织化学法检测CRC组织、癌旁组织(距离肿瘤边缘>5 cm)及淋巴结转移灶中PBX3、E-cadherin和HER-2表达水平,并探讨其与患者临床病理特征及新辅助放射和化学治疗敏感性的关系。结果与癌旁相比,CRC组织中PBX3和HER-2阳性表达率均升高,而E-cadherin阳性表达率降低,差异均有统计学意义(P均<0.05)。TNM分期为Ⅲ~Ⅳ期、有淋巴结转移、有远处转移的患者的CRC组织中PBX3和HER-2阳性表达率均分别高于TNM分期为Ⅰ~Ⅱ期、无淋巴结转移、无远处转移的患者,而E-cadherin阳性表达率则分别低于TNM分期为Ⅰ~Ⅱ期、无淋巴结转移、无远处转移的患者,差异均有统计学意义(P均<0.05)。肿瘤最大径≥5 cm患者的CRC组织中PBX3和HER-2阳性表达率均高于肿瘤最大径<5 cm患者,低分化患者的CRC组织中PBX3阳性表达率高于中~高分化患者,差异均有统计学意义(P均<0.05)。与淋巴结转移灶相比,CRC组织中PBX3阳性表达率降低,差异有统计学意义(P<0.05)。行新辅助放射和化学治疗后,T分期有降期的患者的CRC组织中PBX3阳性表达率高于T分期无降期的患者(P<0.05)。结论CRC组织中PBX3、E-cadherin和HER-2阳性表达率与TNM分期、淋巴结转移均有关,其中PBX3和HER-2阳性表达率均与肿瘤最大径有关,提示三者在CRC病情评估及诊疗中均有重要的临床意义。
Objective This paper intends to investigate the expression and clinical significance of PBX3,E-cadherin,and human epidermal growth factor receptor-2(HER-2)in colorectal cancer tissues and lymph node metastases.Methods The clinical data of 118 patients with colorectal cancer admitted to Yancheng Third People's Hospital from March 2021 to March 2024 were retrospectively analyzed.Immunohistochemistry was used to detect the expression of PBX3,E-cadherin,and HER-2 in cancer tissues,adjacent tissues(>5 cm from the tumor edge),and lymph node metastases.The relationship between PBX3,E-cadherin,HER-2 and clinicopathological characteristics and the sensitivity of neoadjuvant radiotherapy and chemotherapy were analyzed.Results The positive expression rates of PBX3 and HER-2 in colorectal cancer tissues are higher than those in adjacent tissues,while E-cadherin is lower(P<0.05).The positive expression rates of PBX3 and HER-2 in the CRC tissues of patients with TNM stagesⅢtoⅣ,lymph node metastasis,distant metastasis are significantly higher than those of patients with TNM stagesⅠtoⅡ,no lymph node metastasis,and no distant metastasis,while the positive expression rate of E-cadherin is significantly lower than that of patients with TNM stagesⅠtoⅡ,no lymph node metastasis,and no distant metastasis,with statistically significant differences(P<0.05).The positive expression rates of PBX3 and HER-2 in the CRC tissues of patients with the maximum diameter of tumor≥5 cm are higher than in patients with the maximum diameter of tumor<5 cm(P<0.05).The positive expression rate of PBX3 in the tissues of patients with low differentiation of CRC is higher than that in patients with moderate to high differentiation,with statistically significant differences(P<0.05).Compared with lymph node metastatic lesions,the positive expression rate of PBX3 decreases in CRC tissues,with a statistically significant difference(P<0.05).After neoadjuvant radiotherapy and chemotherapy,the positive expression rate of PBX3 in the CRC tissue of patients with T stage reduction is higher than that of patients with no T stage reduction(P<0.05).Conclusions The positive expression rates of PBX3,E-cadherin,and HER-2 in CRC tissues are correlated with TNM stage and lymph node metastasis while the positive expression rates of PBX3 and HER-2 are correlated with the maximum diameter of the tumor.The detection of the above indicators has great clinical significance for evaluating and treating colorectal cancer.
作者
蒋蕾
谷连芬
王婷婷
邱静
JIANG Lei;GU Lianfen;WANG Tingting;QIU Jing(Department of Pathology,the Sixth Affiliated Hospital of Nantong University,Yancheng Third People’s Hospital,Yancheng 224000,China;Department of Pathology,Zhucheng People's Hospital Affiliated to Shandong Second Medical University,Weifang 262200,China)
出处
《国际消化病杂志》
2025年第4期243-248,共6页
International Journal of Digestive Diseases
基金
江苏省老年健康科研项目(LKM2022006)。
