摘要
目的:探讨养脑柔筋方对脑梗死后痉挛性瘫痪大鼠梗死灶周围突触可塑性相关蛋白表达的影响。方法:将雄性SD大鼠随机分为假手术组、模型组、阳性药组及中药组。采用线栓改良法建立大鼠永久性脑梗死模型,通过多通道电生理仪对各组大鼠进行H反射检测,第7、14、21、28天对各组大鼠进行改良神经功能缺损评分(mNSS)、转棒疲劳实验。末次给药后,采集脑组织样本进行HE染色及免疫荧光染色后分析评估大鼠梗死周围区域与突触可塑性相关的突触素(SYN)、突触后致密蛋白95(PSD-95)和突触后支架蛋白1(Homer1)的表达水平。结果:与假手术组比较,模型组大鼠运动能力下降,mNSS评分升高,Hmax/Mmax比值增加,SYN、PSD-95、Homer1蛋白表达显著下降(P<0.01或P<0.05)。与模型组比较,中药组大鼠运动能力显著增加(P<0.01)、mNSS评分显著降低(P<0.01)、Hmax/Mmax比值显著下降(P<0.05),且SYN、PSD-95、Homer1蛋白表达均明显增加(P<0.001)。结论:养脑柔筋方可能通过调控脑梗死后痉挛性瘫痪突触可塑性相关蛋白SYN、PSD-95、Homer1,从而改善大鼠的运动功能障碍。
Objective:To investigate the effects of Yangnao Roujin formula(YNRJF)on the expression of synaptic plasticity-related proteins in the peri-infarct region of rats with post-stroke spasticity(PSS).Methods:Male Sprague-Dawley(SD)rats were randomly divided into sham surgery group,model group,positive control group(baclofen)and YNRJF group.A permanent cerebral infarction model was established using the modified thread-occlusion method.H-reflex testing was performed using a multi-channel electrophysiological system.Modified Neurological Severity Score(mNSS)and rotarod fatigue test were assessed on days 7,14,21,and 28.After the final administration,brain tissues were collected for hematoxylin-eosin(HE)staining and immunofluorescence to analyze the expression levels of synaptic plasticity-related proteins[synapsin(SYN),postsynaptic density-95(PSD-95)and Homer1]in the peri-infarct region.Results:Compared with the sham group,the model group exhibited reduced motor function,increased mNSS scores,elevated Hmax/Mmax ratio,and decreased expression of SYN,PSD-95,and Homer1(P<0.01 or P<0.05).Compared with the model group,the YNRJF group showed improved motor function(P<0.01),reduced mNSS scores(P<0.01),decreased Hmax/Mmax ratio(P<0.05),and markedly increased expression of SYN,PSD-95 and Homer1(P<0.001).Conclusion:YNRJF may ameliorate motor dysfunction in PSS rats by regulating synaptic plasticity-related proteins(SYN,PSD-95 and Homer1).
作者
孙雄星
曾珊珊
吴玲应
林仕高
胡腾辉
王释亮
伍大华
谢乐
SUN Xiongxing;ZENG Shanshan;WU linying;LIN Shigao;HU Tenghui;WANG Shiliang;WU Dahua;XIE Le(Graduate School of Hunan University of Chinese Medicine,Changsha Hunan 410208,China;Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine/The Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine,Changsha Hunan 410006,China)
出处
《中医药导报》
2025年第8期17-23,共7页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(8237153821)
国家自然科学基金青年科学基金项目(82104831)
湖南省科技创新计划项目(2023RC3215)
湖南省自然科学基金面上项目(20241J5236)。
