摘要
目的:旨在分离、鉴定紫皮石斛茎腐病病原菌。方法:采集紫皮石斛患病组织,用组织分离法对其病原菌进行分离、纯化,观察菌落形态特征,并进行柯赫氏法则验证,结合形态学特征及rDNA-ITS序列构建系统发育树,对病原菌进行鉴定。结果:紫皮石斛茎腐病的典型症状为水渍状病斑、叶片黄化、枯萎并脱落,最终导致植株死亡。分离得到的病原菌为层出镰刀菌Fusarium proliferatum,其致病植株与田间观察症状一致。形态学和分子生物学分析显示,菌株ZB-84的特征与层出镰刀菌高度一致,且系统发育分析支持其与该菌种的亲缘关系。结论:该研究首次明确层出镰刀菌为紫皮石斛茎腐病的致病菌,为紫皮石斛茎腐病的防控策略提供了新视角,并为进一步研究该病原菌的致病机制及开发特异性防控技术奠定了基础。
Objective:To isolate and identify the pathogen of stem rot in Dendrobium devonianum.Methods:Diseased tissues of D.devonianum were collected,and the pathogen was isolated and purified using tissue isolation method.Colony morphological characteristics were observed,Koch's postulates were fulfilled to verify pathogenicity,and the pathogen was identified based on morphological characteristics combined with phylogenetic tree constructed using rDNA-ITS sequences.Results:Typical symptoms of stem rot in D.devonianum included water-soaked spots,leaf chlorosis,wilting,and defoliation,ultimately leading to plant death.The isolated pathogen was identified as Fusarium proliferatum,and the symptoms of artificially inoculated plants were consistent with those observed in the field.Morphological and molecular analyses showed that strain ZB-84 was highly consistent with F.proliferatum,and phylogenetic analysis supported its close genetic relationship with this species.Conclusion:This is the first report identifying F.proliferatum as the pathogen of stem rot in D.devonianum.The findings provide new insights for the development of stem rot control strategies,and lay a foundation for further studies on the pathogenic mechanism of the pathogen and the development of specific control technologies.
作者
李朝海
龙锐敏
梁丽燕
周莹
李能波
刘卫红
李信萍
段宝忠
Li Chaohai;Long Ruimin;Liang Liyan;Zhou Ying;Li Nengbo;Liu Weihong;Li Xinping;Duan Baozhong(College of Agriculture and Biology Science,Dali University,Dali,Yunnan 671003,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China;International Joint Laboratory for the Development and Utilization of Traditional Chinese Medicine Resources,Dali,Yunnan 671000,China;Institute of Caulis Dendrobii Longling County,Baoshan,Yunnan 678300,China)
出处
《大理大学学报》
2025年第8期30-35,共6页
Journal of Dali University
基金
云南省生物医药重大科技专项项目(202302AA310029)
云南省院士专家工作站项目(202205AF150026)。
关键词
紫皮石斛
茎腐病
层出镰刀菌
形态特征
系统发育
Dendrobium devonianum
stem rot
Fusarium proliferatum
morphological characteristics
phylogeny
