摘要
目的:人微小RNA-26a(hsa-miR-26a)在众多肿瘤类型中的表达差异备受关注,本研究利用生物信息学工具对其靶基因进行预测,并深入探讨其生物学功能及作用机制。方法:运用TargetScan、miRDB、miRWalk和mirDIP等数据库预测hsa-miR-26a的靶基因并通过求交集的方式筛选基因集;利用TiGER数据库分析这些靶基因在人体各组织器官中的表达分布。采用DAVID在线工具执行基因本体论(GO)富集分析和KEGG信号通路富集分析,以揭示其生物学功能。借助Cytoscape软件及其插件,构建了hsa-miR-26a靶基因相关的蛋白质互作网络,并从中筛选出Hub基因。结果:hsa-miR-26a在肝内胆管癌、胃癌、乳腺癌、肝细胞癌、多发性骨髓瘤、肺癌等多种肿瘤中表达显著下降(P<0.05)。通过Target Score排序筛选出前30个靶基因,这些靶基因在不同组织和器官中的表达量具有统计学差异(P<0.001)。GO富集分析显示,hsa-miR-26a的靶基因主要在细胞质、细胞核及染色质中表达,涉及DNA转录、细胞周期进程、RNA聚合酶转录调控、表观遗传学、有丝分裂细胞周期调控、骨骼发育等生物学过程。KEGG信号通路富集分析显示,这些靶基因在长期抑郁、阿尔茨海默病、mTOR信号通路和肌醇磷酸代谢等路径中高度富集。蛋白质互作网络分析最终确定CCNC、MAML1、CDK8、ADAM17、FBXL19、PLCB1、RHBDF2、RHBDF1为hsa-miR-26a的Hub基因。结论:hsa-miR-26a展现出广泛且复杂的生物学特性。本研究通过多生物信息学技术联合分析,揭示了hsa-miR-26a的靶点及部分生物学功能,为后续实验设计和深入研究提供了重要的可行性依据。
Objective:The expression variances of human microRNA-26a(hsa-miR-26a)in diverse tumor types have drawn considerable attention.The present study aimed to predict its target genes using bioinformatics tools and to comprehensively explore its biological functions and underlying mechanisms.Methods:Multiple databases such as TargetScan,miRDB,miRWalk,and mirDIP were employed to predict the target genes of hsa-miR-26a.The gene sets were then refined by taking the intersection.The TiGER database was utilized to analyze the expression patterns and distributions of these target genes in various human tissues and organs.Gene Ontology(GO)enrichment analysis and KEGG signaling pathway enrichment analysis were carried out via the DAVID online tool to elucidate the biological functions.With the aid of Cytoscape software and its plugins,a protein-protein interaction network related to the target genes of hsa-miR-26a was constructed,and Hub genes were subsequently identified.Results:The expression of hsa-miR-26a was significantly reduced in several tumors,including intrahepatic cholangiocarcinoma,gastric cancer,breast cancer,hepatocellular carcinoma,multiple myeloma,and lung cancer(P<0.05).The top 30 target genes were selected based on Target Score ranking.There were statistically significant differences in the expression levels of these target genes among different tissues and organs(P<0.001).GO enrichment analysis revealed that the target genes of hsa-miR-26a were mainly localized in the cytoplasm,nucleus,and chromatin,and were involved in multiple biological processes such as DNA transcription,cell cycle progression,transcriptional regulation of RNA polymerase,epigenetics,mitotic cell cycle regulation,and skeletal development.KEGG signaling pathway enrichment analysis demonstrated that these target genes were highly enriched in pathways such as long-term depression,Alzheimer's disease,mTOR signaling pathway,and inositol phosphate metabolism.Protein-protein interaction network analysis finally determined CCNC,MAML1,CDK8,ADAM17,FBXL19,PLCB1,RHBDF2,and RHBDF1 as the Hub genes of hsa-miR-26a.Conclusion:Hsa-miR-26a exhibits extensive and complex biological characteristics.Through the integrated utilization of multiple bioinformatics techniques,this study has uncovered the target genes and partial biological functions of hsa-miR-26a,providing an essential and reliable basis for subsequent experimental designs and in-depth research.
作者
王玉婷
张泽高
马苗苗
刘浩
任宏涛
WANG Yuting;ZHANG Zegao;MA Miaomiao;LIU Hao;REN Hongtao(Department of Radiotherapy,People's Hospital of Xinjiang Uygur Autonomous Region,Xinjiang Urumqi 830000,China;Department of Radiotherapy,the Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710000,China)
出处
《现代肿瘤医学》
2025年第10期1719-1726,共8页
Journal of Modern Oncology
基金
新疆维吾尔自治区自然科学基金(编号:2022D01C641)。
