摘要
目的 研究外周血环状RNA微管微丝交联因子1(circMACF1)、微小RNA(miR)-16-5p对心肌梗死(AMI)介入术后心力衰竭的预测价值。方法 选择2022年3月至2024年3月于海南西部中心医院接受介入手术的128例AMI患者,根据术后1年是否发生心力衰竭将128例AMI患者分为心力衰竭组(n=38)和非心力衰竭组(n=90)。比较两组术后即刻外周血circMACF1、miR-16-5p表达水平、血清实验室指标C反应蛋白(CRP)、心肌肌钙蛋白I(cTnI)、N末端B型利钠肽原(NT-proBNP)及心超指标左心室射血分数(LVEF)以及缩短分数(FS)的差异,采用Pearson检验分析circMACF1、miR-16-5p表达水平与CRP、cTnI、NT-proBNP、LVEF、FS的相关性,采用ROC曲线分析circMACF1、miR-16-5p表达水平对术后心力衰竭的预测价值。结果 心力衰竭组的外周血circMACF1表达水平及LVEF、FS低于非心力衰竭组,外周血miR-16-5p表达水平及血清CRP、cTnI、NT-proBNP水平高于非心力衰竭组,差异有统计学意义(P<0.05);外周血circMACF1表达水平与CRP、cTnI、NT-proBNP水平呈负相关,与LVEF、FS呈正相关;miR-16-5p表达水平与CRP、cTnI、NT-proBNP水平呈正相关,与LVEF、FS呈负相关(P<0.05);circMACF1表达水平升高是介入术后心力衰竭的保护因素,miR-16-5p表达水平升高是介入术后心力衰竭的危险因素(P<0.05);外周血circMACF1、miR-16-5p表达水平预测介入术后心力衰竭的AUC分别为0.823和0.809(P<0.05)。结论 circMACF1表达降低、miR-16-5p表达升高与AMI介入术后心力衰竭有关,两项指标对心力衰竭具有预测价值。
Objective To investigate the predictive value of peripheral blood circular RNA microtu⁃bule actin crosslinking factor 1(circMACF1)and microRNA(miR)⁃16⁃5p for heart failure after myocardial in⁃farction(AMI)interventional surgery.Methods A total of 128 AMI patients who underwent interventional surgery at Hainan Western Central Hospital from March 2022 to March 2024 were selected for this study.These patients were then divided into two groups:the heart failure group(n=38)and the non⁃heart failure group(n=90)based on whether they experienced heart failure within one year after surgery.The differences in the levels of circMACF1 and miR⁃16⁃5p in peripheral blood,as well as serum laboratory indicators such as C⁃reactive pro⁃tein(CRP),cardiac troponin I(cTnI),N⁃terminal pro⁃B⁃type natriuretic peptide(NT⁃proBNP),and echocar⁃diogram indicators like left ventricular ejection fraction(LVEF)and shortening fraction(FS)immediately after surgery between the two groups were compared.The correlations between the expression levels of circMACF1 and miR⁃16⁃5p and CRP,cTnI,NT⁃proBNP,LVEF,and FS were analyzed using the Pearson test,and as⁃sessed the predictive value of circMACF1 and miR⁃16⁃5p for postoperative heart failure using ROC curve analy⁃sis.Results The expression level of circMACF1 in peripheral blood,LVEF,and FS in the heart failure group were lower than those in the non⁃heart failure group.Meanwhile,the expression level of miR⁃16⁃5p in peripheral blood and the levels of serum CRP,cTnI,and NT⁃proBNP in the heart failure group were higher than those in the non⁃heart failure group,and the differences were statistically significant(P<0.05).The expression level of circMACF1 in peripheral blood was negatively correlated with the levels of CRP,cTnI,and NT⁃proBNP,and positively correlated with LVEF and FS.Conversely.the expression level of miR⁃16⁃5p was positively correlated with the levels of CRP,cTnI,and NT⁃proBNP,and negatively correlated with LVEF and FS(P<0.05).The in⁃creased expression level of circMACF1 was identified as a protective factor for heart failure after interventional surgery,while the increased expression level of miR⁃16⁃5p was deemed a risk factor for heart failure after inter⁃ventional surgery(P<0.05).The AUC values of the expression levels of circMACF1 and miR⁃16⁃5p in periph⁃eral blood for predicting heart failure after interventional surgery were 0.823 and 0.809,respectively(P<0.05).Conclusion The decreased expression of circMACF1 and the increased expression of miR⁃16⁃5p are associated with heart failure following interventional surgery for AMI.Both markers have predictive value for heart failure.
作者
余秉昌
赵展庆
曾垂旭
赖震宇
蔡秋燕
YU Bingchang;ZHAO Zhanqing;ZENG Chuixu;LAI Zhenyu;CAI Qiuyan(Intensive Care Medicine Department of Hainan Western Central Hospital,Danzhou,Hainan,China,571700)
出处
《分子诊断与治疗杂志》
2025年第8期1570-1573,共4页
Journal of Molecular Diagnostics and Therapy
基金
海南省自然科学项目(820MS154)。
