摘要
目的 研究与早产相关的基因在孕妇血浆中表达量的差异及其预测孕妇早产的应用价值。方法 选取2019年1月至10月于福州市第一医院进行产前常规产检的43例孕妇作为研究对象。将孕妇按年龄段、有无早产史、有无先兆早产症状及分娩情况进行分组,采用基于微流控芯片的实时荧光PCR分析方法检测孕妇血浆中RGS18、DAPP1、RAB27B、PPBP、MOB1B以及MAP3K7CL基因的循环游离RNA(cell-free RNA,cfRNA)表达量。结果 与足月组(满37周)相比,早产组(未满37周)中RAB27B和RGS18基因表达量增加,差异有统计学意义(P<0.05);高危年龄组(≥35岁)中各基因的cfRNA表达量与低危年龄组(<35岁)相比,差异无统计学意义(P>0.05);与无早产史组(未发生过单胎自发性早产)相比,有早产史组(发生过单胎自发性早产)中RAB27B、RGS18和PPBP基因表达量增加,差异有统计学意义(P<0.05);有先兆早产症状组(CL≤20 mm)中各基因的cfRNA表达量与无先兆早产症状组(CL>20 mm)相比,差异无统计学意义(P>0.05)。结论 孕妇血浆中RAB27B、RGS18以及PPBP基因的cfRNA的高表达与早产的发生有一定的相关性,可能存在临床应用价值。
Objective To study the difference in the expression of genes related to preterm birth in pregnant womens plasma and its application value in predicting preterm birth.Methods 43 pregnant women who underwent routine prenatal examinations at Fuzhou First Hospital from January to October 2019 were se⁃lected as the study subjects.They were grouped according to age,delivery status,history of preterm birth,and symptoms of preterm birth.Real⁃time PCR based on microfluidic chips was used to detect the expression of RGS18,DAPP1,RAB27B,PPBP,MOB1B,and MAP3K7CL in the pregnant womens plasma.Results Com⁃pared to the full⁃term group(over 37 weeks),the expression of RAB27B and RGS18 in the preterm birth group(less than 37 weeks)was significantly higher(P<0.05).There was no statistically significant difference in the expression of six genes between the low⁃risk age group(<35)and the high⁃risk age group(≥35)(P>0.05).In comparison to the group with no history of preterm delivery(no history of spontaneous preterm birth in a single⁃ton pregnancy),the expression of RAB27B,RGS18,and PPBP in the group with a history of premature birth(history of spontaneous preterm birth in a single pregnancy)was significantly higher(P<0.05).However,there was no statistically significant difference in the expression of six genes between those with or without symptoms of threatened preterm labor(CL≤20 mm or>20 mm)(P>0.05).Conclusion The increased expression of RAB27B,RGS18,and PPBP in the plasma of pregnant women was found to be associated with preterm birth.This finding may have value in clinical applications.
作者
王颖
詹丽群
齐碧如
WANG Ying;ZHAN Liqun;QI Biru(Obstetrical Department,Fuzhou No.1 Hospital Affiliated with Fujian Medical University,Fuzhou,Fujian,China,350009)
出处
《分子诊断与治疗杂志》
2025年第8期1522-1525,共4页
Journal of Molecular Diagnostics and Therapy
基金
福建省自然科学基金项目[闽科资(2022)19号]
福州市市级临床重点专科建设项目(201610192)。
关键词
早产
循环游离RNA
高通量微流控分析系统
Preterm birth
Cell⁃free RNA
High⁃throughput microfluidic analysis system
