摘要
目的通过转录组学分析细菌性肺炎老年患者与健康老年人差异miRNAs,进行功能富集及通路富集分析。方法选取2023年10月至2024年6月我院呼吸科收治的60岁以上且诊断为细菌性肺炎患者40例为观察组,选取同期我院行健康体检的60岁以上健康人20例为对照组。采用转录组测序,提取两组转录组数据进行差异miRNAs表达分析,差异miRNAs筛选条件为P<0.05且|Log2FC|>1。根据筛选条件P<1×10^(-8)、|Log2FC|>1筛选出差异miRNAs中高置信度差异miRNAs。使用基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)对差异miRNAs进行富集分析。结果通过转录组学测序鉴定出577个差异miRNAs(P<0.05),其中上调miRNAs有405个,下调miRNAs有172个。筛选出ggo-miR-142_R-1、hsa-miR-140-3p_R+1、hsa-miR-424-3p、hsa-miR-3909、mmu-miR-25-5p_R-1、hsa-miR-548am-3p_R-1、ptr-miR-548p_L-3R+2、hsa-miR-548av-3p_L+2R-1_1、hsa-miR-548av-3p_L+2R-1_2、hsa-miR-503-5p_R-2_1ss21CA、hsa-miR-143-3p_R-1、ptr-miR-142_R-112个高置信度差异miRNAs,ptr-miR-142_R-1表达下调,其余表达上调。GO功能富集显示差异miRNAs可通过调控转录因子表达,影响免疫细胞功能。KEGG富集分析提示差异miRNAs与代谢通路相关。结论老年细菌性肺炎高置信度差异miRNAs可作为感染诊断标志物或治疗靶点,富集分析提示差异miRNAs与代谢通路等相关。
Objective To identify the differentially expressed miRNAs between elderly patients with bacterial pneumonia and healthy elderly individuals through transcriptomic analysis,and to conduct functional enrichment and pathway enrichment analyses on these miRNAs.Methods A total of 40 elderly patients(the observation group)diagnosed with bacterial pneumonia and admitted to the respiratory department of our hospital from October 2023 to June 2024,and 20 healthy elderly individuals(the control group)who underwent physical examinations at the same period were selected.Transcriptome sequencing was performed on these samples to obtain the analysis data.The transcriptome data of the bacterial infection group and the healthy group were extracted for differential miRNAs expression analysis.The screening criteria for differential miRNAs were P<0.05 and|Log2FC|>1.High-confidence differential miRNAs were further selected based on the criteria of P<1×10^(-8) and|Log2FC|>1.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used to perform enrichment analysis on the differential miRNAs.Results Through transcriptomic sequencing,a total of 577 differential miRNAs(P<0.05)were identified,including 405 up-regulated miRNAs and 172 down-regulated miRNAs.According to the statistical significance(P<1×10-8 and|Log2FC|>1),12 high-confidence differential miRNAs were selected,including ggo-miR-142_R-1、hsa-miR-140-3p_R+1、hsa-miR-424-3p、hsa-miR-3909、mmu-miR-25-5p_R-1、hsa-miR-548am-3p_R-1、ptr-miR-548p_L-3R+2、hsa-miR-548av-3p_L+2R-1_1、hsa-miR-548av-3p_L+2R-1_2、hsa-miR-503-5p_R-2_1ss21CA、hsa-miR-143-3p_R-1、ptr-miR-142_R-1,which was down-regulated,the others were all up-regulated.GO functional enrichment showed that differential miRNAs may affect the function of immune cells by regulating the expression of transcription factors.KEGG enrichment analysis suggested that the differential miRNAs were related to metabolic pathways.Conclusion The high-confidence differential miRNAs identified in elderly patients with bacterial pneumonia may serve as diagnostic markers or therapeutic targets for infection.Enrichment analysis indicated that these differential miRNAs were associated with metabolic pathways and others.
作者
郭亚文
胡巧
黄朝旺
张静
王丹
赵鹏
陈晓龙
胡明冬
Guo Yawen;Hu Qiao;Huang Chaowang;Zhang Jing;Wang Dan;Zhao Peng;Chen Xiaolong;Hu Mingdong(Department of Geriatrics and Special Services,the Second Affiliated Hospital of Army Medical University,Chongqing 400037,China;Department of Health Management,the Second Affiliated Hospital of Army Medical University,Chongqing 400037,China)
出处
《中华肺部疾病杂志(电子版)》
2025年第4期521-526,共6页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
陆军军医大学科技创新能力提升专项项目(2022XQN33)
陆军军医大学第二附属医院青年博士人才孵育项目(2022YQB065)。
