摘要
目的:结合体内外实验研究拟黑多刺蚁醇提物(PDAEs)抗类风湿关节炎(RA)炎症反应的活性。方法:采用牛Ⅱ型胶原诱导胶原性关节炎大鼠模型和LPS诱导的RAW264.7细胞炎症模型对PDAEs的体内外抗RA炎症反应的作用进行考察。结果:PDAEs在体内和体外均抑制M1型巨噬细胞极化,减少TNF-α、IL-1β、IL-6、iNOS等促炎细胞因子及介质的表达,促进M2型巨噬细胞极化,增强IL-10、TGF-β等抗炎细胞因子表达,从而达到抗炎作用;体内实验表明PDAEs具有免疫调节作用,其潜在机制可能是通过调节PD-1和TGF-β的表达调节Th17/Treg平衡,纠正过强的自身免疫反应。结论:PDAEs可能通过抗炎和免疫调节作用减轻RA的炎症反应。
Objective:To investigate the anti-inflammatory activity of alcohol extract of Polyrhachis dives(PDAEs)against rheumatoid arthritis(RA)in vitro and in vivo.Methods:In vivo and in vitro anti-RA inflammatory response of PDAEs was investigated using a rat model of collagenous arthritis induced by bovine typeⅡcollagen and an LPS-induced RAW264.7 cell inflammatory model.Results:PDAEs inhibited the polarization of M1 type macrophages in vivo and in vitro,reduced expressions of TNF-α,IL-1β,IL-6,iNOS,and promoted polarization of M2 type macrophages,enhanced expressions of anti-inflammatory cytokines,such as IL-10 and TGF-β,so as to achieve the anti-inflammatory effect.The experiments in vivo also showed that PDAEs had the immunomodulatory effect,the potential mechanism may be the regulation of Th17/Treg balance by regulating the expression of PD-1 and TGF-β,thus correcting the over-strong autoimmune response.Conclusion:PDAEs may reduce the inflammatory reaction of RA through anti-inflam-matory and immunomodulatory effects.
作者
何丽丹
赵开军
刘路路
黄子倩
王雨涵
王海丽
HE Lidan;ZHAO Kaijun;LIU Lulu;HUANG Ziqian;WANG Yuhan;WANG Haili(Nanjing Zhongshan Pharmaceutical Co.,Ltd.,Jiangsu Classical Prescriptions Engineering Research Center,Nanjing 210046,China;China Pharmaceutical University,School of Traditional Chinese Pharmacy,Nanjing 211198,China;Jiangsu Hongdian Research Institute of Traditional Chinese Medicine Industry Co.,Ltd.,Nanjing Clinical Experience Prescription Modernization Engineering Research Center,Nanjing 210042,China)
出处
《中国免疫学杂志》
北大核心
2025年第8期1865-1872,共8页
Chinese Journal of Immunology
基金
企业知识产权战略推进计划项目(ZT20210180-33)。
关键词
拟黑多刺蚁
类风湿关节炎
巨噬细胞
抗炎
免疫调节
Polyrhachis dives
Rheumatoid arthritis
Macrophage
Anti-inflammatory
Immunomodulation
