摘要
目的:探究前列腺癌(PC)患者组织联丝蛋白(SYNM)、N-乙酰氨基半乳糖转移酶7(GALNT7)、叉头框转录因子F2(FOXF2)表达与临床病理特征和术后预后的关系。方法:选择2020年3月至2021年9月于武汉科技大学附属华润武钢总医院行前列腺根治术的PC患者112例作为研究对象,记录PC患者的年龄、临床分期、淋巴结转移、肿瘤直径、Gleason评分等临床病理特征,收集PC患者术后癌组织及其癌旁正常组织样本,采用免疫组织化学法检测组织中SYNM、GALNT7、FOXF2表达情况;分析SYNM、GALNT7、FOXF2表达与PC患者临床病理特征的关系;术后随访三年,记录PC患者生存情况,单因素和多因素Cox回归分析影响PC患者术后预后的因素;Kaplan-Meier曲线分析SYNM、GALNT7、FOXF2表达与PC患者3年生存率的关系。结果:SYNM主要表达于细胞质,GALNT7在细胞膜和细胞质均有表达,FOXF2主要定位于细胞核。SYNM在观察组的阴性率为82.14%(92/112),显著高于对照组的11.61%(13/112),χ^(2)=111.883,P<0.001;GALNT7在观察组的阳性率为85.71%(96/112),显著高于对照组的4.46%(5/112),χ^(2)=149.315,P<0.001;FOXF2在观察组的阴性率为80.36%(90/112),显著高于对照组的16.07%(18/112),χ^(2)=92.690,P<0.001。PC患者中,临床分期晚、有淋巴结转移、肿瘤直径大、Gleason评分高及术前PSA水平高者,其SYNM阴性率、GALNT7阳性率、FOXF2阴性率均显著高于临床分期早、无淋巴结转移、肿瘤直径小、Gleason评分低及术前PSA水平低者(P<0.05)。PC患者术后死亡与年龄、临床分期无关(P>0.05),与肿瘤直径、术前PSA水平、Gleason评分、淋巴结转移有关(P<0.05);术前PSA水平、Gleason评分、SYNM阴性表达、GALNT7阳性表达、FOXF2阴性表达均是PC患者3年内死亡的重要影响因素(P<0.05);SYNM阳性表达患者3年生存率显著高于阴性表达患者(χ^(2)=24.633,P<0.05),GALNT7阴性表达患者3年生存率高于阳性表达患者(χ^(2)=16.453,P<0.05),FOXF2阳性表达患者3年生存率明显高于其阴性表达患者(χ^(2)=24.977,P<0.05)。结论:PC患者组织SYNM、GALNT7、FOXF2的表达与临床分期、淋巴结转移、肿瘤直径、Gleason评分、术前PSA水平密切相关,SYNM和FOXF2阴性表达、GALNT7阳性表达均是PC患者术后预后的重要影响因素。
Objective:To investigate the association of tissue-linked mitogenin(SYNM),N-acetylaminogalactosyltransferase 7(GALNT7),and forkhead box transcription factor F2(FOXF2)expression with clinicopathological features and postoperative prognosis in patients with prostate cancer(PC).Methods:A total of 112 cases of PC patients who underwent radical prostatectomy at CR WISCOM General Hospital affiliated to Wuhan University of Science and Technology from March 2020 to September 2021 were selected as study subjects;clinicopathological features such as age,clinical stage,lymph node metastasis,tumour diameter,Gleason score and so on were recorded;samples of cancer tissues and their paracancerous normal tissues of the PC patients were collected after the operation,and immunohistochemistry was used to detect SYNM,GALNT7,FOXF2 expression in the tissues;the relationship between SYNM,GALNT7,FOXF2 expression and clinicopathological characteristics of PC patients was analysed;three years of postoperative follow-up was conducted to record the survival of PC patients,and the factors affecting the postoperative prognosis of PC patients were analysed by unifactorial and multifactorial Cox regression;the SYNM was analysed by Kaplan-Meier curve,GALNT7,FOXF2 expression in relation to 3-year survival of PC patients.Results:SYNM was mainly expressed in the cytoplasm,GALNT7 was expressed in both the cell membrane and cytoplasm,and FOXF2 was mainly localised in the nucleus.The negative rate of SYNM in the observation group was 82.14%(92/112),which was significantly higher than that of the control group[11.61%(13/112)],(χ^(2)=111.883,P<0.001);the positive rate of GALNT7 in the observation group was 85.71%(96/112),which was significantly higher than that of 4.46%(5/112)in the control group(χ^(2)=149.315,P<0.001);the negative rate of FOXF2 in the observation group was 80.36%(90/112),which was significantly higher than that of 16.07%(18/112)in the control group(χ^(2)=92.690,P<0.001).Among the PC patients,those with late clinical stage,lymph node metastasis,large tumour diameter,high Gleason score and high preoperative PSA level had significantly higher SYNM-negative rate,GALNT7-positive rate,and FOXF2-negative rate than those with early clinical stage,no lymph node metastasis,small tumour diameter,low Gleason score,and low preoperative PSA level(P<0.05).The prognostic death of PC patients was not related to age and clinical stage(P<0.05),but was related to tumour diameter,preoperative PSA level,Gleason score,and lymph node metastasis(P<0.05);preoperative PSA level,Gleason score,negative expression of SYNM,GALNT7-positive expression,and FOXF2-negative expression were all significant influencing factors for death within 3 years in PC patients(P<0.05);the 3-year survival rate was significantly higher in patients with SYNM-positive expression than in patients with negative expression(χ^(2)=24.633,P<0.05).The 3-year survival rate in patients with GALNT7-negative expression was higher than in patients with positive expression(χ^(2)=16.453,P<0.05).The 3-year survival rate of patients with positive expression of FOXF2 was significantly higher than that of patients with negative expression(χ^(2)=24.977,P<0.05).Conclusion:The expression of SYNM,GALNT7,and FOXF2 in the tissues of PC patients is closely related to clinical stage,lymph node metastasis,tumor diameter,Gleason score,and preoperative PSA level.Negative expression of SYNM and FOXF2,and positive expression of GALNT7 are important factors affecting the postoperative prognosis of PC patients.
作者
黎书豪
伍庄
吕艳
何露
邓飞
LI Shuhao(Medical College,Wuhan University of Science and Technology,Hubei Wuhan 430081,China)
出处
《河北医学》
2025年第8期1302-1309,共8页
Hebei Medicine
基金
2021年湖北省科技创新专项基金项目,(编号:2021CFB537)。
