摘要
鸟类在自然生态系统和人类社会发展中扮演着重要角色,鸟类的性别鉴定技术对于生态学研究和畜牧业的可持续发展至关重要。传统性别鉴定方法如翻肛法,普遍受限于操作人员的经验及熟练度,存在效率低等问题。近年来,分子性别鉴定技术,包括PCR技术、分子标记技术和恒温扩增技术在内,已得到迅速发展。这些技术的应用提高了鸟类性别鉴定的准确性和简便性,尤其是恒温扩增技术的出现,加速了鸟类性别鉴定技术的发展,成为鸟类性别鉴定中的新型工具。与PCR和分子标记技术相比,恒温扩增技术以其高效性、特异性好、操作简单的特点在鸟类性别鉴定中展现出显著优势。恒温扩增技术在单一温度下反应30~60 min即可完成待测样品扩增,达到检测水平。笔者回顾了常规性别鉴定方法,并以环介导恒温扩增技术(loop-mediated isothermal amplification,LAMP)、重组酶聚合酶恒温扩增技术(recombinase polymerase amplification,RPA)/重组酶介导恒温扩增技术(recombinase-aided polymerase amplification,PAA)和交叉引物恒温扩增技术(cross-priming amplification,CPA)在鸟类性别鉴定中的应用为例,阐述不同恒温扩增技术在鸟类性别鉴定中的研究进展,为后续研究提供参考。
Birds play a crucial role in both natural ecosystems and human societies,and the technology for identifying bird sex is vital for ecological research and the sustainable development of animal husbandry.Traditional sex identification methods,such as anal swelling,are generally limited by the experience and proficiency of the operators,and by low efficiency.In recent years,molecular sex identification technology,including PCR,molecular markers technology,and isothermal amplification technology have advanced rapidly.These applications have improved the accuracy and simplicity of sex identification in birds.Especially the introduction of isothermal amplification technology has accelerated the development of the sex identification technology and become a new tool in the sex identification of birds.Compared with PCR and molecular markers techniques,isothermal amplification has shown significant advantages in bird sex identification with its high efficiency,specificity and simple operation.Isothermal amplification technology can complete sample amplification and reach the detection level in 30 to 60 minutes at a single temperature.The authors reviewed the conventional methods of sex identification,using loop-mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA)/recombinase-aided polymerase amplification(PAA),and cross-priming amplification(CPA)in sex of birds as examples,illustrating the progress of the different isothermal amplification techniques in the sex identification of birds,and providing reference for future related research.
作者
谭深滦
曾繁文
李莉
卫恒习
张守全
TAN Shenluan;ZENG Fanwen;LI Li;WEI Hengxi;ZHANG Shouquan(National Engineering Research Center for Breeding Swine Industry,Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University,Guangzhou 510642,China;Guangzhou Zoo/Guangzhou Wildlife Research Center,Guangzhou 510075,China)
出处
《中国畜牧兽医》
北大核心
2025年第9期4237-4247,共11页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(32272873)。
