摘要
茶树(Camellia sinensis)是具有重要经济价值的木本作物,低温冻害易使茶树的产量和品质受到严重损害。CsWRKY29转录因子参与植物的冷适应过程,但是该基因在经济作物烟草(Nicotiana tabacum)中的利用价值及其在糖信号交叉的反应过程中的作用还不是很清楚。本研究通过烟草叶盘转化法和RT-qPCR技术,获得了过表达CsWRKY29基因的转基因烟草植株。通过对低温胁迫下的生理生化指标进行分析,包括离子渗漏率、超氧化物歧化酶活性、丙二醛含量、可溶性糖含量,明确了过表达CsWRKY29的烟草可能通过增加可溶性糖积累和提高抗氧化物酶活性,降低低温下的膜脂伤害,从而增强植物的抗寒性。利用酵母单杂交实验发现,CsWRKY29能够直接与茶树CsSUS4基因的启动子区域结合。该结果表明过表达CsWRKY29的植物可能通过激活CsSUS4从而启动糖信号途径,在低温下积累更多的可溶性糖,进而影响植物的抗寒能力。另外,本研究发现过表达CsWRKY29在一定程度上影响了转基因烟草生殖生长期的株高及叶片大小。该结果暗示CsWRKY29在调控植物生长发育方面可能具有负调控作用。本研究为深入理解CsWRKY29在植物生长发育平衡和逆境响应中的复杂生物学功能提供了重要的理论依据。
Tea plant(Camellia sinensis)is a woody crop of significant economic value,and its yield and quality can be severely damaged by freezing injury.The CsWRKY29 transcription factor is involved in the plant′s cold adaptation process,but the utility of this gene in economically important crops like tobacco and its role in the cross-reactive sugar signaling pathway are not well understood.In this study,transgenic tobacco plants(Nicotiana tabacum)overexpressing the CsWRKY29 gene were obtained using the tobacco leaf disc transformation method and RT-qPCR technology.Physiological and biochemical indicators under cold stress were analyzed,including ion leakage rate,superoxide dismutase activity,malondialdehyde content,and soluble sugar accumulation,revealing that the transgenic tobacco overexpressing CsWRKY29 may enhance plant cold resistance by increasing soluble sugar content and improving antioxidant enzyme activity,thereby reducing membrane lipid damage under low temperature.Yeast one-hybrid experiments demonstrated that CsWRKY29 can directly bind to the promoter region of the tea plant CsSUS4 gene.This result suggests that overexpression of CsWRKY29 in tobacco may activate the sugar signaling pathway,leading to the accumulation of more soluble sugars under low temperatures,thereby affecting the plant′s cold resistance.Additionally,this study found that overexpression of CsWRKY29 somewhat affected the plant height and leaf size of transgenic tobacco during the reproductive growth period,implying a potential negative regulatory role of CsWRKY29 in controlling plant growth and development.This research provides important theoretical evidence for a deeper understanding of the complex biological functions of CsWRKY29 in balancing plant growth and development and responding to stress.
作者
鲁力
陈娅
薛承进
黄小贞
赵懿琛
LU Li;CHEN Ya;XUE Chengjin;HUANG Xiaozhen;ZHAO Yichen(College of Tea Sciences,Guizhou University,Guiyang,550025;Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences,Guizhou University,Guiyang,550025)
出处
《基因组学与应用生物学》
北大核心
2025年第6期527-536,共10页
Genomics and Applied Biology
基金
黔科合科技支撑[2023]一般012项目
国家自然科学基金项目(31960615,32160077)共同资助。
关键词
茶树
低温胁迫
生长发育
转录因子
酵母单杂交
Camellia sinensis
Cold stress
Growth and development
Transcription factor
Yeast one-hybrid
