摘要
以毛竹叶为原料,采用原位泡腾-机械化学(SEMC)法提取7种酚类成分,包括新绿原酸、绿原酸、隐绿原酸、异荭草苷、荭草苷、牡荆苷和异牡荆苷,利用高分辨质谱结合超高效液相色谱对竹叶中7种供试酚类化合物进行定性和定量分析,采用扫描电子显微镜对研磨前后竹叶样品的形貌结构进行比较。在单因素试验基础上,进一步通过正交试验优化提取工艺,结果表明:最佳提取工艺为研磨时间180 s、提取时间180 s、碳酸氢钠150 mg、液料比12∶1(mL∶g)。在优化条件下,毛竹叶和毛竹实生苗叶中7种供试酚类成分的提取总得率分别为6.00和7.12 mg/g。机械研磨可以使大颗粒样品变为小尺寸颗粒,造成细胞结构破坏,促进酚类物质的释放。本研究建立的原位泡腾-机械化学法具有高效、快速等特点,优于传统冷浸(CME)法和超声波辅助提取(UAE)法,适合于竹叶样品中主要酚类成分的提取。
An in situ effervescence reaction-assisted mechanochemical extraction(SEMC)method was used to extract seven phenolic compounds,including neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isoorientin,orientin,vitexin and isovitexin,in moso bamboo leaves.The qualitative and quantitative analysis of seven phenolic compounds in bamboo leaves was carried out by high resolution mass spectrometry and ultra-high performance liquid chromatography.The morphology and structure of the bamboo samples before and after milling were compared by scanning electron microscope.Based on single factor tests,the extraction process was further optimized by means of orthogonal tests.The results showed that the optimal extraction process was as follows:milling time of 180 s,extraction time of 180 s,sodium bicarbonate amount of 150 mg,liquid-solid ratio of 12∶1(mL∶g).Under the optimal conditions,the total rates of seven phenolic compounds from the leaves and seedlings of moso bamboo were 6.00 and 7.12 mg/g,respectively.Mechanical milling turns large samples into small-sized particles,resulting in the destruction of cell structure and promoting the release of phenolic compounds.The proposed method has the characteristics of high efficiency and rapid,which is superior to the traditional cold maceration extraction(CME)and ultrasound assisted extraction(UAE),and is suitable for the extraction of main phenolic compounds from bamboo leaves.
作者
高赫然
张道褀
王进
汤锋
GAO Heran;ZHANG Daoqi;WANG Jin;TANG Feng(Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo&Rattan Science and Technology,International Centre for Bamboo and Rattan,Beijing 100102,China)
出处
《林产化学与工业》
北大核心
2025年第4期90-96,共7页
Chemistry and Industry of Forest Products
基金
国家重点研发计划资助项目(2022YFD2200602)。
关键词
黄酮
酚酸
机械化学法
泡腾
提取工艺
竹叶
flavonoids
phenolic acids
mechanochemical method
effervescence
extraction process
bamboo leaves
