摘要
以木姜叶柯老叶为原料,采用超声波辅助法提取根皮苷,以根皮苷得率为指标,在单因素试验基础上,通过Box-Behnken响应面试验优化根皮苷提取工艺。建立游离脂肪酸(FFA)诱导的L02细胞胰岛素抵抗模型,以葡萄糖消耗和糖原水平为指标,探讨根皮苷体外减轻胰岛素抵抗的能力。研究结果表明:木姜叶柯老叶中根皮苷的最佳提取工艺为乙醇体积分数60%、提取时间45 min、提取温度60℃、超声波功率70 W、液料比20∶1(mL∶g),在该条件下根皮苷得率为10.05%。将木姜叶柯老叶75%乙醇提取物经大孔树脂洗脱、正向硅胶柱和十八烷基硅烷键合硅胶(ODS)柱纯化后,得到纯度大于98%的根皮苷样品。此外,根皮苷能够促进胰岛素抵抗L02细胞中葡萄糖的消耗及糖原合成,起到减轻胰岛素抵抗的作用。当根皮苷浓度为100μmol/L时,葡萄糖消耗为5.13 mmol/L,与阳性对照二甲双胍(MET)的5.19 mmol/L接近;糖原水平为83.81%,明显高于MET的68.43%。
Phlorizin was extracted from Lithocarpus litseifolius mature leaves via ultrasound-assisted extraction.The extraction process was optimized using a Box-Behnken response surface design based on single-factor experiments,with phlorizin yield as the primary evaluation criterion.Additionally,a free fatty acid(FFA)-induced insulin-resistant L02 hepatocyte model was established to investigate the potential of phlorizin in ameliorating insulin resistance,with glucose consumption and glycogen level as key metabolic indicators.The optimal conditions for phlorizin extraction were determined as follows:ethanol concentration(60%,V/V),extraction time(45 min),temperature(60℃),ultrasonic power(70 W),and solvent-to-material ratio(20∶1,mL∶g).Under these conditions,the phlorizin yield reached 10.05%.The 75% ethanol extract of L.litseifolius mature leaves was sequentially purified using macroporous resin,normal-phase silica gel chromatography,and reverse-phase ODS column chromatography,yielding phlorizin with a purity exceeding 98%.Phlorizin significantly enhanced glucose consumption and glycogen synthesis in insulin-resistant L02 cells.At a concentration of 100μmol/L,phlorizin increased glucose consumption to 5.13 mmol/L,comparable to the positive control metformin(MET,5.19 mmol/L).Notably,phlorizin elevated glycogen level to 83.81%,markedly higher than MET(68.43%).
作者
潘峰
赵恒
吴雪
杨韩
王皓雪
付玉杰
PAN Feng;ZHAO Heng;WU Xue;YANG Han;WANG Haoxue;FU Yujie(Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040,China;College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,China;College of Forestry,Beijing Forestry University,Beijing 100083,China)
出处
《林产化学与工业》
北大核心
2025年第4期10-18,共9页
Chemistry and Industry of Forest Products
基金
北京林业大学“5·5工程”科研创新团队项目(BLRC2023A01)
高等学校学科创新引智计划项目(B20088)
黑龙江省“头雁”计划林木遗传育种创新团队项目(无编号)。
关键词
木姜叶柯
根皮苷
响应面优化
胰岛素抵抗
Lithocarpus litseifolius(Hance)Chun
phlorizin
response surface optimization
insulin resistance
