摘要
微生物除臭是一种有效的恶臭废气处理技术,菌种对生物除臭效果具有决定性的影响。【目的】筛选可降解二甲基二硫醚(dimethyl disulfide,DMDS)的菌株,并探究其在不同条件下的降解效果及产表面活性剂的能力。【方法】以含硫恶臭有机物DMDS为唯一碳源,从红树林底泥中筛选可自产生物表面活性剂并能降解DMDS的菌株R1。利用生理生化特性分析和16S rRNA基因测序对菌株进行鉴定,利用红外光谱和核磁分析确定自产生物表面活性剂的种类。【结果】通过生理生化特性及16S rRNA基因序列分析鉴定,菌株R1属于木糖氧化无色杆菌(Achromobacter xylosoxidans)。研究表明,在DMDS初始浓度为12.49 mg/L、温度为30℃、接种量为1.0 g/L的条件下,DMDS的降解率可达70.74%。乳化实验表明,菌株R1能够利用DMDS作为碳源分泌生物表面活性剂,核磁和红外光谱分析鉴定表明,菌株R1产生的表面活性剂种类为糖脂类。【结论】DMDS降解的中间产物主要为甲硫醇,硫元素的转化率为65.99%。菌株R1表现出良好的降解能力和产表面活性剂性能。
Microbial deodorization is an effective technology for treating odorous waste gases,in which microbial strains play a decisive role.[Objective]To screen the strains capable of degrading dimethyl disulfide(DMDS)and investigate their degradation efficiency and ability to produce surfactants under various conditions.[Methods]DMDS,a typical sulfur-containing odorous organic compound,was selected as the sole carbon source.A strain R1 capable of simultaneously producing biosurfactants and degrading DMDS was isolated from mangrove sludge.The strain was identified based on the physiological and biochemical characteristics analysis and 16S rRNA gene sequencing.The types of self-produced biosurfactants were determined using infrared spectroscopy and nuclear magnetic resonance spectroscopy analysis.[Results]Based on physiological and biochemical characteristics and 16S rRNA gene sequence,strain R1 was identified as Achromobacter sp.The strain was capable of degrading DMDS,with optimal degradation conditions of an initial DMDS concentration of 12.49 mg/L,a system temperature of 30℃,and an inoculum amount of 1.0 g/L,under which the DMDS degradation rate reached 70.74%.Emulsification experiments showed that strain R1 can use DMDS as a carbon source to produce biosurfactants,which were identified as glycolipids through nuclear magnetic resonance and infrared spectroscopy.[Conclusion]The main intermediate product in the biodegradation of DMDS is methyl mercaptan,and the transformation rate of sulfur to SO42-is 65.99%.Strain R1 exhibits impressive performance in degrading DMDS and producing biosurfactants.
作者
吴江丽
孙蕾
袁丹
万顺刚
WU Jiangli;SUN Lei;YUAN Dan;WAN Shungang(School of Ecology,Hainan University,Haikou,Hainan,China;School of Chemistry and Chemical Engineering,Hainan University,Haikou,Hainan,China)
出处
《微生物学报》
北大核心
2025年第8期3615-3629,共15页
Acta Microbiologica Sinica
基金
海南省自然科学基金(425RC684)
国家自然科学基金(32060291)。
关键词
二甲基二硫醚
生物降解
分离鉴定
生物表面活性剂
除臭
dimethyl disulfide
biodegradation
isolation and identification
biosurfactant
deodorization
