摘要
以2,2'-联噻吩-5-甲醛和7-(二乙胺基)香豆素-3-甲酰肼为原料,合成了一种基于香豆素-噻吩类新型荧光探针L,并通过核磁、红外、质谱以及高分辨质谱对其进行结构表征。在甲醇溶液中,探针L对Cu^(2+)的选择识别能力较高,识别Cu^(2+)后,体系的荧光强度减弱40倍,且检测过程中可避免常见阳离子和阴离子的干扰。荧光滴定实验表明,探针L对Cu^(2+)的检测具有较高的灵敏度,当Cu^(2+)的浓度在0~5μmol·L^(-1)范围时,体系的荧光强度与Cu^(2+)的浓度呈直线关系,检测限低至2.6×10^(-9)mol·L^(-1)。通过Job's plot曲线和高分辨质谱证明了探针L与Cu^(2+)的络合比为2:1。将探针L作为试纸使用,实现了对Cu^(2+)的“裸眼”检测,表明该探针在环境监测领域有一定的实用价值。
A novel coumarin-thiophene-lmsed fluorescent probe L was synthesized using 2,2'-Bithiophene-5-oarboxaldehyde and 7-(Diethylaniino)coumarin-3-carhohydrazide as resource materials,and its structure was characterized by NMR.FT-IR.MS and HUMS.In methanol solution,probe L Had high selective recognition ability for Cu^(2+).After recognizing Cu^(2+),the fluorescence intensity of the system was weakened by 40 times,and interference from common cations and anions could he avoided during the detection process.Fluorescence titration experiments showed that probe L had high sensitivity for the detection of Cu^(2+).When the concentration of Cu^(2+)was in the range of 0~5μmol·L^(-1).the fluorescence intensity of the system showed a linear relationship with the concentration of Cu^(2+).and the detection limit was as low as 2.6×10^(-9)mol·L^(-1).The Job's plot curve and HUMS confirmed that the complexation ratio of probe L with Cu^(2+)was 2:1.Using probe L as a test strip,the“naked-eye”detection of Cu^(2+)was achieved,indicated that the probe had potential application in the environmental monitoring field.
作者
熊新
介百佳
刘芳雅
张迎乐
王知逸
何梦宇
舒朋华
XIONG Xin;JIE Bai-jia;LIU Fang-ya;ZHANG Ying-le;WANG Zhi-yi;HE Meng-yu;SHU Peng-hua(College of Food and Pharmacy,Xuchang University,Xuchang 461000,China)
出处
《化学研究与应用》
北大核心
2025年第8期2229-2236,共8页
Chemical Research and Application
基金
河南省自然科学基金项目(252300420811)资助
河南省科技厅科技攻关项目(242102320275)资助
河南省高等学校重点科研项目(25B550017)资助
国家级大学生创新创业训练计划项目(202410480019)资助。
