摘要
以香豆素醛和芘腙为原料经缩合反应合成了配体ABX,它分别对Cd^(2+)和Cu^(2+)有着完全不同的紫外和荧光响应。竞争实验表明,若有Cu^(2+)的存在则会对探针识别Cd^(2+)造成干扰,Cu^(2+)会与ABX-Cd^(2+)发生置换反应生成更为稳定的ABX-Cu^(2+)配合物。因此,着重考察了ABX与Cd^(2+)络合生成的ABX-Cd^(2+)对Cu^(2+)的识别性能。结果表明,探针ABX-Cd^(2+)可高选择、高灵敏度识别Cu^(2+),荧光响应效率可由ABX直接识别Cu^(2+)的68.71%显著提升至96.25%。紫外光下负载探针ABX-Cd^(2+)的“试纸”呈橙红色,再浸入不同浓度的Cu^(2+)后荧光逐渐猝灭,从而成功应用于对自来水和工业废水中Cu^(2+)的半定量检测;此外,在紫外灯照射下用ABX溶液在硅胶板上书写的黄绿色字母经涂Cd^(2+)后变橙红色,然后进一步涂Cu^(2+)后荧光猝灭,该识别效果适于应用在加密防伪油墨领域。探针ABX-Cd^(2+)也被应用于细胞内对Cu^(2+)的生物成像研究。
In this paper,the ligand ABX was prepared by condensation reaction of coumarin aldehyde and pyrene hydrazone.The probe has completely different UV and fluorescence responses towards Cd^(2+)and Cu^(2+),respectively.Competitive experiments showed that the presence of Cu^(2+)interferes with the Cd^(2+)recognition performance by probe ABX,and the substitution reaction between Cu^(2+)and ABX-Cd^(2+)produceed a more stable ABX-Cu^(2+)complex.Therefore,the recognition performance of ABX-Cd^(2+)complex for Cu^(2+)was investigated.The results showed that the probe ABX-Cd^(2+)could identify Cu^(2+)with high selectivity and sensitivity,and the fluorescence response efficiency was significantly improved from 68.71%to 96.25%compared with that of Cu^(2+)directly identified by ABX.The“test paper”of the loaded probe ABX-Cd^(2+)was orange-red under ultraviolet light,and then the fluorescence was gradually quenched after being immersed in Cu^(2+),so it was successfully applied to the semi-quantitative detection of Cu^(2+)in tap water and industrial wastewater.In addition,the letters written with ABX solution on the silica gel were yellowish green under ultraviolet light and then became orange-red after applying Cd^(2+),and the fluorescence was completely quenched after continuing to apply Cu^(2+),which was suitable for application in the field of encryption and anti-counterfeiting ink.Most importantly,The probe ABX-Cd^(2+)has also been successfully applied to the bioimaging studies of intracellular Cu^(2+).
作者
肖嘉扬
朱鑫玥
郭小云
杨帆
杨泰然
陈蕊
高云
高妍
XIAO Jiayang;ZHU Xinyue;GUO Xiaoyun;YANG Fan;YANG Tairan;CHEN Rui;GAO Yun;GAO Yan(College of Chemical Engineering,University of Science and Technology Liaoning,Anshan 114051,China;Yingkou Food and Drug Testing Center,Yingkou 115003,China)
出处
《发光学报》
北大核心
2025年第8期1526-1535,共10页
Chinese Journal of Luminescence
基金
国家自然科学基金(U1910215)
辽宁省自然科学基金面上项目(2024LNYKJ27)
国家级大学生创新创业训练计划项目(202410146032)
辽宁省大学生创新创业训练计划项目(S202410146065)。
